烟草及烟草制品中霉菌数量检验用培养基的开发与应用  

Development and Application of Medium for Tobacco Mold Counting Test

作　　者：林华清[1] 曾令杰[1] 王嘉乐[1] 沙云菲[1] 戚大伟[1] 吴达[1] 

机构地区：[1]上海烟草集团有限责任公司

出　　处：《烟草科技》2014年第11期29-32,共4页Tobacco Science & Technology

基　　金：国家烟草专卖局标准项目"烟草及烟草制品霉变的判定"(2013QB020)

摘　　要：为开发适用于烟草及烟草制品中霉菌数量检验用的培养基,以烟草及烟草制品中8种常见的污染霉菌(黄曲霉、黑曲霉、局限曲霉、桔青霉、产黄青霉、总状毛霉、匍枝根霉和绿色木霉)为试验菌种,采用5种常见的霉菌培养基(孟加拉红琼脂、察氏琼脂、高盐察氏琼脂、含20%蔗糖的高渗察氏琼脂和马铃薯葡萄糖琼脂培养基)及4种研发的新型培养基进行烟草及烟草制品中霉菌数量检验用培养基的初筛和复筛研究。结果表明:与其他培养基相比,Ⅰ号改良马铃薯葡萄糖琼脂培养基(马铃薯200 g,葡萄糖20.0 g,NaCl 30.0 g,琼脂16.0 g,氯霉素0.1 g和蒸馏水1000 mL)具有检验霉菌菌相全面、获得霉菌数量结果准确等优点,适用于烟草及烟草制品中霉菌数量的检测。In order to develop a medium suitable for testing the amount of mold,eight molds common in tobacco and tobacco products were chosen （including Aspergillus flavus,A.niger ATCCl6404,A.restrictus CGMCC 3.3973,Penicillium citrinum,P.chrysogenum,Mucor racemosus CGMCC 3.206,Rhizopus stolonifer CGMCC 3.2045 and Trichoderma viride）,five common mold media （Rose Bengal agar,Czapek agar,High salt Czapek agar,hypertonic Czapek agar containing 20％ sucrose,and Potato dextrose agar） and four pilot media were tested via primary and secondary screening.The results showed that comparing with other media,the improved medium,potato dextrose agar No.1 （potato 200 g,glucose 20.0 g,NaCl 30.0 g,agar 16.0 g,chloramphenicol 0.1 g and distilled water 1000 mL）,was more suitable for tobacco mold counting test due to its coverage of microflora and accurate counting results.

关 键 词：烟草 烟草制品 霉菌数量检验 培养基 

分 类 号：TS414[农业科学—烟草工业]