阿尼芬净关键中间体棘白菌素B的加压毛细管电色谱分析  

Analysis of anidulafungin key immediate echinocandin B by pressurized capillary electrochromatography

作　　者：赵琪[1] 彭沪[2] 闵涛玲[1] 胡海峰[1] 

机构地区：[1]中国医药工业研究总院上海医药工业研究院,创新药物与制药工艺国家重点实验室,上海200040 [2]上海市第十人民医院急救中心,上海200040

出　　处：《中国抗生素杂志》2014年第11期830-832,共3页Chinese Journal of Antibiotics

基　　金：由国家重大科学仪器设备开发专项(No.2011YQ15007208);国家"重大新药创新"科技重大专项(No.2012ZX09301002-003)资助

摘　　要：目的 建立抗真菌药物-阿尼芬净关键中间体棘白菌素B（Echinocandin B,ECB）加压毛细管电色谱分析（pCEC）方法.方法 采用流动相为乙腈-4.35mmol/L的磷酸盐缓冲盐（pH 5.5）（52∶48,V/V）,等度洗脱,流速为0.08mL/min,紫外检测波长212nm,分离电压-15kV.结果 各组分在0.015～3.84mg/mL范围内线性良好;检出限和定量限分别为15和30μg/mL;日间和日内精密度均小于5％;加样回收率均高于98％.结论 该方法简单方便,重现性好,准确可靠,回收率较高,为阿尼芬净中间体ECB的分析和质量控制提供了新的方法.Objective A pCEC method was established for the effìcient determination of the key immediate echinoeandin B （ECB） of antifungal Anidulafungin.Methods The method was used with the mobile phase of acetonitrile-phosphate buffer （4.35 mmol/L,pH5.5）（52∶48,V/V） at flow rate of 0.08mL/min,separation voltage of～15kV and the detection wavelength of 212nm.Results The calibration curve for ECB was linear in the concentration range of 0.015～3.84mg/mL.The determination limit and quantitative limits were 15 and 30μg/mL,respectively.The inner-day and inter-day precisions were all less than 5%.All recoveries were more than 98%.Conclusion This new method was simple,rapid and reproducible,and can be applied to the quantitative analysis of echinocandin B.

关 键 词：加压毛细管电色谱 棘白菌素B 阿尼芬净 抗真菌 

分 类 号：R917[医药卫生—药物分析学]