芎汤有效部位对人肝癌细胞BEL-7402活力的影响  

Effect of Active Part of Xiongqiong Decoction on Vitality of BEL-7402 Human Hepatocellular Carcinoma Cells

作　　者：郭小藤[1] 赵睁睁[1] 容蓉[1] 杨勇[2] 胡金芳[1] 

机构地区：[1]山东中医药大学药学院,山东济南250355 [2]山东中医药大学基础医学院,山东济南250355

出　　处：《山东中医药大学学报》2014年第6期587-590,共4页Journal of Shandong University of Traditional Chinese Medicine

基　　金：山东省自然科学基金资助项目(编号:ZR2009CM055);"重大新药创制"科技重大专项资助项目(编号:2010ZX09401-302-4-11)

摘　　要：目的 :研究中药复方芎汤提取物及含药血清对人肝癌细胞BEL-7402活力的影响。方法 :分别给SD大鼠灌服芎汤超临界萃取部位(SFE)和大孔树脂分离得到的20%、40%乙醇洗脱部位(EE20、EE40部位),获得各部位含药血清,设置含药血清组、提取物组、顺铂对照组、空白对照组分别作用于人肝癌细胞BEL-7402,用MTT法测定各组对人肝癌细胞BEL-7402活力的影响。结果:芎汤EE20含药血清在低浓度时抑制作用明显,并随着浓度增高抑制作用减弱;EE40部位含药血清随着药物浓度的增大对癌细胞增殖作用也越来越强,SFE部位含药血清对细胞无显著性作用;提取物对细胞的作用趋势与含药血清基本一致,但含药血清作用强于提取物。结论:芎汤EE20有效部位在低浓度时对人肝癌细胞BEL-7402的增值有一定的抑制作用,为其抗肿瘤作用的进一步研究提供了依据。Objective：To research the effect of active part of Xiongqiong decoction and its medicine-contained serum on the vitality of BEL-7402 human hepatocellular carcinoma cells. Methods：Fed SD rats with SFE part,EE20 part and EE40 part separated by Macroporous resin of Xiongqiong decoction respectively to get medicine-contained serum of every part. The BEL-7402 human hepatocellular carcinoma cells were acted upon by medicine-contained serum,extract,Cisplatin and RPMI-1640. The effect on vitality of BEL-7402 human hepatocellular carcinoma cells was detected by MTY method. Results：The inhibitory effect of serum containing EE20 of Xiongqiong decoction was significant at low concentration and it weakened as the concentration increased. The effect of EE40-contained serum on the proliferation of cancer cells strengthened as the drug concentration increased. The SFE-contained serum had no significant effect on cells. The effect of extract on cells was basically the same as that of the medicine-contained serum,but it was weaker than that of the medicine-contained serum. Conclusion：The active part EE20 of Xiongqiong decoction had some inhibitory effect on the proliferation of BEL-7402 human hepatocellular carcinoma cells at low concentration. This provides a basis for the study of its anti-tumor effect.

关 键 词：芎汤 MTT法 提取物 含药血清 肝癌细胞 

分 类 号：R285.5[医药卫生—中药学]