小麦育种亲本材料SSR标记遗传多样性及其亲缘关系分析  

Analysis of Genetic Diversity and Relationship among Wheat Breeding Parents by SSR Markers

作　　者：王升星[1] 朱玉磊[1] 张海萍[1] 常成[1] 马传喜[1] 

机构地区：[1]安徽农业大学农学院/农业部黄淮南部小麦生物学与遗传育种重点实验室,安徽合肥230036

出　　处：《麦类作物学报》2014年第5期621-627,共7页Journal of Triticeae Crops

基　　金：国家自然科学研究基金项目(31000705);现代农业产业技术体系建设专项(nycytx-03);农业部行业科研专项(201203033-04)项目;安徽省小麦产业技术体系项目

摘　　要：为了解不同生态区小麦品种间的遗传多样性,以190份分布于黄淮冬麦区、长江中下游冬麦区及西南冬麦区3大麦区的小麦品种为试验材料,利用均匀分布于小麦21条染色体的80对SSR标记对其进行遗传多样性研究,并进行品种间亲缘关系的分析。本文共检测出352个等位类型,每个标记平均4.4个;各位点的多态性信息含量的变幅分别为0.021 2-0.853 2,平均为0.533 4。结果表明：3大麦区品种间遗传差异较为明显,其中长江中下游冬麦区与西南冬麦区遗传差异相对较小,而黄淮冬麦区西部丘陵川地副区与胶东丘陵副区存在较大差异。各麦区间及副区间都存在不同程度的品种交叉现象,其中黄淮冬麦区华北平原副区与淮北平原副区间的种质资源交换较为频繁。遗传聚类结果与品种间亲缘关系相对一致,与品种系谱来源及地域分布也较为吻合,即同一麦区或者具有共同系谱来源的小麦品种可较好地聚为一类。本文研究结果为小麦亲本选配提供了有用的遗传信息和重要参考依据。To understand the genetic diversity of wheat varieties widely planted in different wheat regions in china.A set of 80SSR markers uniformly distributed on 21wheat chromosomes was used to analyze the genetic diversity and relationship among 190wheat cultivars mainly from three main winter wheat regions including Yellow Huai River,middle and lower reaches of the Yangtze River, and Southwestern.Totally 352alleles were detected,and the average alleles per locus were 4.4.The polymorphism information content（PIC）value ranged from 0.021 2to 0.853 2,with an average of 0.533 4.The results showed that a significant genetic difference was observed among wheat varieties from the three main wheat regions;thereinto,smaller difference was found between that from the Middle and lower reaches of the Yangtze River and Southwestern winter wheat regions,while bigger difference was found between that from the West upland sub-region of Yellow Huai River and Jiaodong upland sub-region.Exchange of wheat germplasm was also found among different wheat regions,and high frequency was observed in North China plain sub-region and North Huai River plain sub-region of Yellow Huai river winter wheat regions.The result of cluster analysis by SSR mark-ers was coincident with their genetic relationship,also closely matching with their pedigrees and regional distribution.Those wheat varieties originated from the same pedigrees or wheat regions were clustered together.The results provided important information for selecting the parent materials during wheat breeding.

关 键 词：小麦 遗传多样性 SSR标记 

分 类 号：S512.1[农业科学—作物学] S330