海带渣降解液中糠醛类物质的去除及对乙醇产率影响分析  

Analysis of influence on ethanol yield by removal of furfural in kelp residue degradation

作　　者：吴佐浩[1] 臧家业[2] 董永胜[1] 杨晓[3] 金滨滨[4] 丁慧[3] 赵倩[4] 王能飞[2] 

机构地区：[1]齐鲁工业大学食品与生物工程学院,山东济南250353 [2]国家海洋局第一海洋研究所,山东青岛266061 [3]青岛大学化学化工与环境学院,山东青岛266071 [4]成都中医药大学药学院,四川成都611130

出　　处：《可再生能源》2014年第11期1753-1758,共6页Renewable Energy Resources

基　　金："十二五"国家科技计划课题(2011BAD14B04);中央级公益性科研院所基本科研业务费专项资金(2011G25);南北极环境综合考察与评估专项(CHINARE2013-02-01);海洋公益性行业科研专项(201005031)

摘　　要：利用色谱检测以及葡萄糖溶液模拟发酵,确定了稀酸预处理后海带渣纤维素酶酶解所得糖液中糠醛(2-F)、5-羟甲基糠醛(5-HMF)的含量及其对酵母发酵产乙醇的影响。数据分析结果表明,酶解糖液中的2-F和5-HMF浓度分别为132.4 mg/L和158.5 mg/L,其中2-F对发酵有微弱的促进作用,5-HMF使乙醇产率下降8%。通过蒸发、吸附、萃取、碱化等方法对酶解糖液进行脱毒处理,对比脱毒前后发酵情况发现,Ca(OH)2碱化处理对2-F和5-HMF的去除率分别达到94.4%和77.7%。发酵还原糖乙醇转化率提高14.6%,产率提高10.9%。By using chromatographic detection and simulation experiment with glucose solution, we determine the concentrations of furfural and 5-hydroxymethylfurfural in eellulase enzymatic hy- drolysate of kelp residue that pretreated by dilute acids, and the influence of the two materials on the yeast fermentation to produce alcohol. The results showed that the concentrations of furfural and 5 - hy- droxymethylfurfural in the cellulase enzymatic hydrolysate were 132.4 mg/L and 158.5 mg/L ,however, the furfural could promote the fermentation slightly hut the 5-hydroxymethylfurfural fell ethanol pro- duction rate by 8%. In addition, through several detoxification methods that included evaporation, ad- sorption, extraction, basification act on cellulase enzymatic hydrolysate, comparing the fermentation re- sult of detoxificating or not, we found the basification of Ca （OH）2 could remove 94.4% of furfural and 77.7% of 5-hydroxymethylfurfural. The conversion rate of reducing sugar to ethanol enhanced 14.6% and the yield enhanced 10.9%.

关 键 词：海带渣 色谱 酸预处理 糠醛 5-羟甲基糠醛 乙醇 发酵 

分 类 号：TK6[动力工程及工程热物理—生物能] TQ352.6[化学工程]