启动子对蓝藻合成乙醇能力影响的研究  

Study on the effect of different promoters on ethanol producing capacity with Cyanobacteria

作　　者：高政绪 刘中庆[1] 刘宏[1] 刘秀丽 王德权[1] 张英华[1] 高倩倩[2,4] 杜传印[1] 

机构地区：[1]山东潍坊烟草有限公司,山东潍坊261205 [2]中国科学院青岛生物能源与过程研究所,中国科学院生物燃料重点实验室,山东青岛266101 [3]昌乐县农业局,山东昌乐262400 [4]临沂市科学技术合作与应用研究院,山东临沂276002

出　　处：《可再生能源》2014年第11期1759-1765,共7页Renewable Energy Resources

基　　金：企业合作项目(20113702001142)

摘　　要：从集胞藻PCC6803中克隆了Prbp1,Prnpb和PnrtA3个启动子,用于控制蓝藻中乙醇合成相关酶的表达,分别构建了3个基因工程藻株Syn-ZG72,Syn-ZG73和Syn-ZG74。在相同培养条件下,与之前构建的携带Prbc启动子的Syn-ZG25相比,Syn-ZG73乙醇产量与之相当,明显高于Syn-ZG72和Syn-ZG74,表明Prnpb的表达效果强于Prbp1和PnrtA。Prbp1不能控制乙醇的诱导合成,PnrtA可以控制乙醇的诱导合成,但是启动子强度弱,乙醇产量低。CO2 can be converted to biofuel molecules by genetic engineered Cyanobacteria. Bioethanol is one of significantly biofuel molecules. More enzymes related with ethanol synthesis would produce more ethanol. Three Cyanobacteria strains Syn-ZG72 Syn-ZG73 and Syn-ZG74 were constructed by carrying Prrbp1 ,Pmpb and Pn,A of Synechocystis sp. PCC 6803, respectively. Compared with Syn-ZG25 car- rying Prbc, the ethanol production of Syn-ZG73 and Syn-ZG25 were very close and higher than Syn- ZG72 and Syn-ZG74 under the same condition. The results demonstrated that the expression level of Pmpb and Prbc were very close and higher than Prbpl and PnrtA. Prbp1 could not induce the ethanol producing. In contrast, Pn, A could induce the ethanol producing, but the intensity of this promoter was weak and the ethanol production was low.

关 键 词：蓝藻 集胞藻PCC6803 启动子 乙醇 

分 类 号：TK6[动力工程及工程热物理—生物能] Q291[生物学—细胞生物学]