猪繁殖与呼吸综合征病毒分离株GP5蛋白ORF5基因的克隆与序列分析  

Cloning and Sequence Analysis of GP5 Genes of Porcine Reproductive and Respiratory Syndrome Virus Prevalence Strains

作　　者：吴晓薇[1] 刘晓慧 杨谦[3] 熊蕊 郭凤柳 郭霄峰[3] 阳佑天[3] 陈茹[1] 

机构地区：[1]广东出入境检验检疫局,广东广州510642 [2]保定出入境检验检疫局,河北保定071051 [3]华南农业大学,广东广州510640

出　　处：《中国畜牧兽医》2014年第11期40-43,共4页China Animal Husbandry & Veterinary Medicine

基　　金：广东省科技计划项目(2011B020306004)

摘　　要：为揭示广东地区猪繁殖与呼吸综合征(porcine reproductive and respiratory syndrome,PRRS)可能的流行规律,本研究采用RT-PCR的方法扩增5株来自广东地区猪场临床样品的猪繁殖与呼吸综合征病毒(porcine reproductive and respiratory syndrome virus,PRRSV)分离株的GP5蛋白ORF5基因,将其克隆、测序后与国内外部分毒株相应基因进行核苷酸序列比较。结果表明,PRRSV-2011-GD2、PRRSV-2011-GD3、PRRSV-2013-GD1、PRRSV-2013-GD2和PRRSV-2013-GD3株与美洲型参考株(VR-2332)的核苷酸序列同源性分别为88.8%、99.5%、88.7%、88.7%和83.5%。系统进化树分析结果表明,PRRSV-2011-GD2、PRRSV-2011-GD3、PRRSV-2013-GD1、PRRSV-2013-GD2和PRRSV-2013-GD3株均属于美洲型。以上结果为更好地了解PRRSV的分子流行病学特征和抗原变异规律提供依据,同时为研制基因工程疫苗奠定了基础。In order to reveal the Guangdong region of porcine reproductive and respiratory syndrome （PRRS） epidemic law, 5 strains of swine clinical samples amplified from Guangdong region of PRRSV GP5 genes of the isolates with the method of RT- PCR, cloned, sequenced and domestic and foreign strains corresponding gene nucleotide sequence comparison in this study. The results showed that PRRSV-2011-GD2, PRRSV-2011-GD3, PRRSV-2013-GDl, PRRSV-2013-GD2 and PRRSV-2013- GD3 strains with the American type reference strains （ATCC VR-2332 U87392） nucleotide sequence homology were 88. 8 % , 99. 5 %, 88. 7 %, 88. 7 % and 83. 5 % ,respectively. The phylogenetic tree analysis indicated that PRRSV-2011-GD2, PRRSV- 2011-GD3, PRRSV-2013-GD1, PRRSV-2013-GD2 and PRRSV-2013-GD3 strains belonged to the American. The results provided the basis for a better understanding of the molecular epidemiology of PRRSV features and antigenic variation law, also laid the foundation for the study of gene engineering vaccine.

关 键 词：猪繁殖与呼吸综合征 GP5蛋白 序列分析 

分 类 号：S854.43[农业科学—临床兽医学]