伪狂犬病病毒BJ/YT株毒力相关基因gE和TK序列分析  

Sequences Analysis of Virulence Associated Genes gE and TK of Pseudorabies Virus Strain BJ/YT

作　　者：钟承[1] 刘蕾 张乐天[1] 王巨实[1] 王安琦[1] 陆宝生[1] 杨万莲[1] 吕艳丽[1] 

机构地区：[1]中国农业大学动物医学院,北京100193 [2]中国牧工商(集团)总公司,北京100070

出　　处：《中国畜牧兽医》2014年第11期73-79,共7页China Animal Husbandry & Veterinary Medicine

基　　金：中国农业大学动物医院基金

摘　　要：本试验对2012年从北京地区约克夏犬体内分离得到的伪狂犬病病毒（PRV）BJ/YT株主要毒力基因gE和TK的分子特征和进化关系进行了分析。结果显示,BJ/YT株gE基因与参考序列的核苷酸和氨基酸同源性分别为98.9%～100.0%和98.3%～100.0%;TK基因核苷酸和氨基酸同源性分别为99.2%～99.9%和99.0%～100.0%。BJ/YT株与同期河北猪源分离株进化关系较近,各毒株之间同源性高,并且存在一致的核苷酸突变位点;与其他参考序列比对分析结果显示,BJ/YT株主要毒力基因存在变异位点,但这些位点均不在已知的主要功能区和抗原表位区内。因此,从分子流行病学角度来看,BJ/YT毒株是近年北京及其周边地区PRV流行毒株,但gE和TK基因的变异对流行毒株的毒力无明显影响。To analyze the molecular characterization and evolutionary relationship of the virulence associated genes of the BJ/YT strain which was isolated from a Yorkshire Terrier in Beijing in 2012. The results revealed that the homologies of the gE gene nucleotide sequences and amino acid sequences were 98. 9% to 100.0% and 98. 3% to 100.0%, respectively; meanwhile, the homologies of the TK gene nucleotide sequences and amino acid sequence were 99.2% to 99.9% and 99.0% to 100.0%, respectively. The phylogenetic tree based on gE gene showed that BJ/YT and the strains isolated from Hebei province could be grouped into a relatively independent branch, and all of them shared a high homology. Besides, a concordant mutation site was presented. Few mutation sites in gE and TK amino acid sequences were observed when compared with other referenced sequences. But those sites were not located in the main domains or antigenic epitope regions. Therefore, from the view of molecular epidemiology, it was likely that the BJ/YT isolate was the main circulating strain in Beijing and its surrounding area the last few years. However, the variations of gE and TK genes did not have obvious effects on the virulence of the epidemic strain during this period.

关 键 词：伪狂犬病病毒 BJ/YT株 GE基因 TK基因 序列分析 

分 类 号：S852.659[农业科学—基础兽医学]