重组鸡α干扰素/白细胞介素-2融合蛋白对鸡外周血T淋巴细胞亚群的影响研究  

The Influence for Chickens' T Lymphocyte Subsets in Peripheral Blood after Inoculated with the Recombinant Chicken Interferon-α/Chicken Interleukin-2 Complex Protein

作　　者：杨晓璐[1,2] 闫若潜[2] 吴志明[2] 刘梅芬[2] 张代宝[3] 张书阳[1,2] 王淑娟[2] 赵雪丽[2] 刘毅[1,2] 

机构地区：[1]河南科技大学动物科技学院,河南洛阳471003 [2]河南省动物疫病预防控制中心,河南郑州450008 [3]郑州市动物疫病预防控制中心,河南郑州450008

出　　处：《中国畜牧兽医》2014年第11期175-178,共4页China Animal Husbandry & Veterinary Medicine

基　　金：河南省科技创新人才计划项目(豫科人组[2011]1号)

摘　　要：为了研究重组鸡α干扰素/白细胞介素-2融合蛋白（rChIFN-α-Linker-ChIL-2,重组融合蛋白）对SPF鸡外周血T淋巴细胞亚群含量影响,本研究采用流式细胞术对重组融合蛋白（第2组）和rChIFN-α蛋白（第3组）注射14日龄SPF鸡后不同时间对各试验组鸡外周血T淋巴细胞（PBLC）中CD3^＋CD4^＋、CD3^＋CD8^＋百分含量以及CD4^＋/CD8^＋比值进行检测。结果表明,与对照组相比,重组融合蛋白和rChIFN-α蛋白在鸡体内接种后3～14d均可明显提高鸡外周血T淋巴细胞中CD3^＋CD4^＋细胞百分含量,降低CD3^＋CD8^＋细胞的百分含量,提高CD4^＋/CD8^＋比值;接种后3～7d期间,第2组鸡的CD3^＋CD4^＋、CD3^＋CD8^＋细胞含量及CD4^＋/CD8^＋比值与PBS对照组（第1组）相比差异均极显著（P〈0.01）,第3组与第1组相比差异均显著（P〈0.05）。以上结果表明,重组融合蛋白和rChIFN-α蛋白接种鸡体后均可显著影响鸡PBLC中淋巴细胞亚群百分含量,提高CD4^＋/CD8^＋比值,增强机体的细胞免疫功能;重组融合蛋白对鸡淋巴细胞亚群百分含量、CD4^＋/CD8^＋比值影响程度和细胞免疫功能的增强作用优于rChIFN-α蛋白;重组融合蛋白在体内发挥了鸡α干扰素和白细胞介素-2对细胞免疫功能的协同增强作用。To explore the influence of recombinant chicken interferon-α/ chicken interleukin-2 fusion protein （rChIFN-α-Linker-ChIL-2 protein. recombinant fusion protein） on the percentage content of peripheral T lymphocyte subsets （PBLC） of SPF chicken. the flow cytometry was used to detected the percentage contents of CD3^＋ CD4^＋, CD3^＋ CD8^＋ and the CD4^＋ /CD8^＋ ratio in PBLC at different days post injection in the 14-day-old SPF chicken injected with rChIFN-α-Linker-ChIL-2 protein （group 2） and recombinant chicken interferon α （rChIFN-α） protein （group 3）. The results indicated that the recombinant fusion protein and rChIFN-α protein could increase the percentage content of CD3^＋ CD4^＋ T lymphocyte. decrease the percentage content of CD3^＋ CD8^＋ T lymphocyte. improve CD4^＋ /CD8^＋ T lymphocyte ratio during 3 to 14 d post injection. The percentage contents of CD3^＋ CD4^＋, CD3^＋ CD8^＋ and CD4^＋ /CD8^＋ ratio in chickens of group 2 were significantly different from the PBS control group （group 1） （P〈0.01） , and were notably different from group 3 during 3 to 7 d post injection （P〈0. 05）. These results showed that both the rChIFN-α-Linker-ChIL-2 protein and rChIFN-α protein could affect the percentage contents of lymphocyte subsets. improve CD4^＋ /CD8^＋ ratio. enhance the cellular immune function in chicken. The influences of rChIFN-α-Linker-ChIL-2 protein on the percentage contents of CD3^＋ CD4^＋ and CD3^＋ CD8^＋ T lymphocyte. and CD4^＋ /CD8^＋ value were notable higher than rChIFN-α. which suggested that recombinant fusion protein playa synergistic role of interferon alpha and interleukin-2 on the cellular immune function by this pathway in chicken.

关 键 词：重组鸡α干扰素/白细胞介素-2融合蛋白 重组鸡α干扰素蛋白 T淋巴细胞亚群 CD4^+/CD8^+比值 免疫功能 

分 类 号：Q45[生物学—生理学]