益气健脾中药对腹泻仔猪小肠黏膜组织结构的影响  

Effect of Tonifying Qi-Nourishing Spleen Herbs on Small Intestinal Mucosa in Diarrheal Piglets

作　　者：赵娟[1] 白元生 武果桃[1] 张东玲[3] 王树华[1] 杨忠 任杰[1] 孟冬霞[1] 孟帆[1] 刘一飞[1] 

机构地区：[1]山西省农业科学院畜牧兽医研究所,山西太原030032 [2]山西省生态畜牧产业管理站,山西太原030001 [3]山西省农业科学院农业资源与经济研究所,山西太原030006 [4]山西省畜牧遗传育种中心,山西太原030009

出　　处：《中国畜牧兽医》2014年第11期252-257,共6页China Animal Husbandry & Veterinary Medicine

基　　金：山西省科技攻关项目(20110311032;20140311020-4)

摘　　要：为了研究益气健脾中药对腹泻仔猪小肠黏膜结构的影响,试验用抗生素及中药复方颗粒剂治疗腹泻仔猪,颈静脉放血处死后,腹腔解剖,无菌采集小肠黏膜,通过制作石蜡切片、HE染色观察小肠黏膜病理组织学变化;电镜包埋切片观察小肠黏膜细胞结构的变化。结果显示,中药复方颗粒剂治疗组小肠黏膜除有少量出血外,未发现明显病理变化。抗生素治疗组则小肠黏膜脱落,有广泛充血、出血;黏膜上皮细胞变性坏死;嗜酸性粒细胞浸润,有大量以淋巴细胞(LELs)、杯状细胞(GSs)为主的炎性细胞浸润;微绒毛排列紊乱、脱落;细胞受损严重,线粒体等细胞器结构异常。结果表明,抗生素治疗仔猪腹泻造成小肠黏膜明显的病理变化和功能障碍,而中药复方颗粒剂治疗有利于仔猪小肠黏膜结构的修复,为防制仔猪腹泻、提高仔猪的生长性能提供理论依据。To investigate the effect of Tonifying Qi-Nourishing Spleen herbs on small intestinal mucosa in diarrheal piglets, antibiotics and Chinese herbal medicine were adopted to heal diarrheal piglets. Piglets were sacrificed by bloodletting in veins, dissected peritoneal cavity, and gathered small intestinal mucosa abacterially. Then, the changes of histopathology and cellular structures in small intestinal mucosa were observed by using pathological and electron microscopic sections. The results showed that small intestinal mucosa had no obvious pathological changes except slight hemorrhage in Chinese herbal medicine group. Nevertheless, there were more pathological changes in antibiotics group, for instance, small intestinal mueosa had desquamated with extensive hyperemia and hemorrhage concomitantly; epithelial ceils were degenerated and necrotic;eosinophilic granulocyte displayed cellular infiltration, which included inflammatory cell infiltrate principally as lymphoeyte;microvillus had desquamated and arranged indiscriminately; mucous membrane cells were damaged seriously and mitochondria presented structural abnormalities. We concluded that the therapy of using antibiotics in diarrheal piglets might cause pathological changes and functional impairment in small intestinal mucosa, and using Chinese herbal medicine might be beneficial for plerosis of small intestinal mucosa, which would provide the theoretical foundation to cure diarrhea and improve growth performance of piglets.

关 键 词：中药 仔猪 小肠黏膜 病理变化 电镜观察 

分 类 号：S853.7[农业科学—临床兽医学]