新技术在聚酮组合生物合成中的应用研究进展  

New Technologies in Polyketide Recombinatorial Biosynthesis

作　　者：黄传龙[1] 谭树华[1] 顾觉奋[1] 

机构地区：[1]中国药科大学生命科学与技术学院,南京210009

出　　处：《国外医药（抗生素分册）》2014年第6期241-245,共5页World Notes on Antibiotics

摘　　要：本文综述了生物信息学软件、光交联技术、异源表达以及底盘细胞在聚酮组合生物合成中的应用。并尝试将其整合为一粗略的开发思路,即"计算机虚拟设计→高通量技术筛选→(底盘细胞作宿主)异源表达"。希望通过引入新技术,能进一步提高聚酮组合生物合成的开发效率。Several new technologies-including bioinformatic soflwares, photocrosslinking, heterologous expression and cellular chassis, and their applications in polyketide recombinatorial biosynthesis, were introduced in this paper. We also tried to integrate these technologies into a new route for improving efficiency of the development of polyketide recombinatorial biosynthesis, that is ＂fictitious design in computer →verifying by high-throughput technologies→heterologous expression（ by cellular chassis ）＂.

关 键 词：聚酮 组合生物合成 ClustScan 光交联 异源表达 底盘细胞 

分 类 号：R98[医药卫生—药品]