铜锈环棱螺HSP70对Cd和BDE-47胁迫的响应敏感性  

Response Sensitivity of HSP70 in Bellamya aeruginosa Exposed to Cadmium and BDE-47

作　　者：马陶武[1] 王萌[1] 彭巾英[1] 何兴兵[1] 吴玲玲[2] 龙奕[1] 刘珊珊[1] 

机构地区：[1]吉首大学生物资源与环境科学学院,湖南吉首416000 [2]同济大学环境科学与工程学院,上海200092

出　　处：《重庆师范大学学报（自然科学版）》2014年第6期22-28,共7页Journal of Chongqing Normal University:Natural Science

基　　金：国家自然科学基金项目(No.41171383);生态旅游湖南省重点实验室开放基金项目(No.JDSTLY1409)

摘　　要：采用沉积物生物毒性测试,分别研究了不同含量Cd和BDE-47污染沉积物对铜锈环棱螺（Bellamya aeruginosa）肝胰脏热休克蛋白HSP70表达水平的变化,以揭示Cd和BDE-47胁迫与HSP70表达响应的时间/剂量-效应关系,探讨HSP70作为持久性有毒物质对铜锈环棱螺早期伤害生物标志物的敏感性。结果表明,受到不同含量Cd和BDE-47加标沉积物胁迫后,铜锈环棱螺肝胰脏HSP70表达均表现出较为明显的时间/剂量-效应关系。在Cd短期（0~10 d）暴露下,HSP70应激水平随Cd含量的升高而显著升高（p〈0.05）;在Cd长期（10~21 d）暴露下,HSP70应激水平随Cd含量的升高表现为先升后降,低Cd含量（5μg·g^-1）暴露下HSP70应激水平随暴露时间的延长而显著上升（p〈0.05）;中、高Cd含量（25、100μg·g^-1）暴露下HSP70应激水平随时间的延长均呈先升后降的趋势。不同含量BDE-47暴露下HSP70应激水平时间的延长大体上均呈先升高后降低的变化趋势;低、中含量BDE-47（160、640 ng·g^-1）短期暴露对HSP70应激水平没有影响,而长期（10~21 d）暴露后,HSP70应激水平均显著降低（p〈0.05）。比较而言,HSP70对Cd低含量（5μg·g^-1）短期（低于10 d）暴露显示出较好的敏感性,而对BDE-47低含量（160 ng·g^-1）短期暴露表现不敏感;低含量Cd和BDE-47长时间（10~21 d）暴露下,HSP70均具有较好的指示作用。The expression levels of hepatopancreatic heat shock protein 70 （HSP70） in Bellamya aeruginosa exposed to Cd- and BDE-47-contaminated sediments, respectively, through sediment toxicity bioassay to reveal the time- and dose-effect relationships between Cd/BDE-47 exposure and HSP70 expression, and to further explore the sensitivity of HSP70 as a biomarker indicating the early damage of persistent toxic substances to B. aeruginosa. The results indicated that hepatopanereatic HSP70 expression in B. aeruginosa after exposure to Cd- or BDE-47-spiked sediments with various contents showed distinct time- and dose-dependent effect relationships. In the case of short-term （0~10 d） Cd exposure, HSP70 expression levels showed significant increase in a dose-de pendent manner; but within relatively long-term （10~21 d） Cd exposure stage, HSP70 expression levels displayed a trend of first increase and then decrease with increasing Cd content. Cd exposure with low content （5 μg · g^-1） caused a time-dependent increase of HSP70 expression, and Cd exposure with medium and high contents （25 and 100 μg · g^-1 ） led to initial increase and subsequent decrease of HSP70 expression with prolonged exposure duration. On the whole, HSP70 expression levels in all BDE-47 treatment groups showed first increase and then decrease with prolonged exposure duration. In the case of short term （0~10 d） BDE-47 expo- sure with low and medium contents （160 and 640 ng · g ^-1）, HSP70 expression levels did not show significant differences compared with that of the controls, however, after relatively long-term （10~21 d） BDE-47 exposure, HSP70 expression levels decreased sig- nificantly. Comparatively, HSP70 showed relatively good sensitivity responding to short-term exposure （〈10 d） with low Cd con tent （5 μg· g^-1）, but indicated insensitivity responding to short-term exposure with low BDE-47 content （160 ng · g^-1 ）. Under long-term （10N21 d） Cd or BDE-47 exposure with low contents, HSP70 can be u

关 键 词：CD BDE-47 铜锈环棱螺 肝胰脏 沉积物 HSP70 生态毒性 生物标志物 

分 类 号：Q958.116[生物学—动物学]