肠道真菌过增殖小鼠模型的建立  

Establishment of a Mice Model of Fungal Over-growth in Gastrointestine

作　　者：唐欢[1] 李凡[2] 魏泓[3] 

机构地区：[1]重庆中国三峡博物馆,重庆400015 [2]重庆市中医院肾病科,重庆400011 [3]第三军医大学基础部实验动物学教研室,重庆400038

出　　处：《重庆师范大学学报（自然科学版）》2014年第6期116-119,F0003,共5页Journal of Chongqing Normal University:Natural Science

基　　金：国家"973"计划课题(No.2013CB531406)

摘　　要：通过给予头孢曲松造成SPF级BALB/c小鼠(Mus musculus)发生肠道菌群紊乱,进而通过连续灌胃密度为2×107CFU/m L的白假丝酵母(Candida albicans)菌液以建立小鼠肠道真菌过增殖模型;通过镜检、活菌计数、结肠组织病理切片、电镜观察等方法评价白假丝酵母在小鼠体内黏附定植的情况。实验结果显示,大剂量头孢曲松处理后,活菌计数结果显示小鼠肠道菌群出现重度失衡;灌胃真菌后,镜检可见酵母相和菌丝相的白假丝酵母,PAS染色可见小鼠结肠粘膜表面黏附有大量染成红色的圆形真菌孢子;扫描电镜观察发现小鼠结肠表面黏附大量卵圆型白假丝酵母的酵母相细胞。研究认为大剂量抗生素处理结合真菌灌胃,可成功建立小鼠肠道真菌过增殖模型。To establish a mice model with intestinal fungal infection, 50 mg/d ceftriaxone was given to the SPF BALB/c mice intra- gastrically for 5 clays and next the Candida albicans was inoculated in order to simulate the over-growth of fungi. Changes of mice fecal flora were observed by optical microscope and SEM directly. Enumerations of intestinal microflora and the elimination of Can- dida albicans were detected by vital cell counting. Treatment with ceftriaxone at dosage of 50 mg/d would result in a severe damage of murine intestinal microflora. After Candida albicans were given intragastrically for 5 days, both yeast and hyphal cells of Candi- da albicans could be detected in the feces of the mice. Both pathological and SEM detection showed that Candida albicans grew as spherical yeast cells to the mice colonic mucus. So a mice model of fungal over-growth in gastrointestine could be induced by inocula- tion with Candida albicans after treated with ceftriaxone.

关 键 词：肠道菌群 肠源性感染 白假丝酵母 动物模型 

分 类 号：Q954.3[生物学—动物学]