PRRSV XH-GD株NSP7缺失感染性克隆的构建  

Construction and identification of NSP7-deficient clone of porcine reproductive and respiratory syndrome virus(PRRSV)XH-GD strain

作　　者：谢杰雄[1] 崔甜甜[1] 陈耀[1] 张民泽[1] 崔进[1] 查云峰 孙龙[1] 赵孟孟[1] 邓胜朝 张桂红[1] 

机构地区：[1]华南农业大学兽医学院,广东广州510642 [2]广东动物卫生监督研究所,广东广州510230

出　　处：《中国兽医杂志》2014年第9期6-9,共4页Chinese Journal of Veterinary Medicine

基　　金：现代农业产业技术体系建设专项资金资助(CARS-36)

摘　　要：PRRSV的非结构蛋白NSP7是病毒的一种高度保守的非结构蛋白,NSP7可以进一步水解产生NSP7α,NSP7β。目前对于NSP7的研究相对较少,对其生物学功能尚不清楚。为了进一步了解PRRSV NSP7对于病毒的复制的作用,本试验通过反向遗传平台在PRRSV XH-GD全长病毒骨架中分别对NSP7α,NSP7β,NSP7编码区进行缺失,构建NSP7以及NSP7部分缺失株的全长cDNA的重组质粒,并对构建的重组质粒进行病毒拯救。结果表明,对NSP7α,NSP7β,NSP7缺失对病毒的拯救是致死性的,推测NSP7对于病毒的复制具有重要作用,本研究结果对于NSP7的相关功能研究提供一定的参考依据。Nonstructural protein 7（NSP7）is one of the most conserved nonstructural proteins in porcine reproductive and respi-ratory syndrome virus（PRRSV）.It can be further cleaved into NSP7α and NSP7β.The major role of NSP7 remains unclear. To furtheridentify the function of NSP7 in PRRSV,reverse genetic manipulation was performed and an infectious PRRSV cDNA clone with sev-eral NSP7-deficient mutants were constructed to verify the function of NSP7.The results revealed that the PRRSV clones deficient ofNSP7α,NSP7β or NSP7,can not be rescued suggesting that NSP7 is critical to the replication of PRRSV.These results may provide the basis for the functional study of this nonstructural protein.

关 键 词：PRRSV NSP7 缺失构建 

分 类 号：S858.28[农业科学—临床兽医学]