PCR法检测活毒疫苗中禽白血病病毒污染  

Study on PCR Method for the detection of Avian Leukemia Virus Pollution in Live Vaccines

作　　者：胡晓苗[1] 沈学怀[1] 戴银[1] 赵瑞宏[1] 侯宏艳[1] 潘孝成[1] 周学利[1] 张丹俊[1] 朱传明[1] 

机构地区：[1]安徽省农业科学院畜牧兽医研究所,安徽合肥230031

出　　处：《中国兽医杂志》2014年第9期23-25,共3页Chinese Journal of Veterinary Medicine

基　　金：国家现代农业产业技术体系项目(CARS-41);安徽省农业科学院科技创新团队项目(11C0404);安徽省科技攻关项目(11010302119);国家自然科学基金项目(31302044)

摘　　要：为调查活毒疫苗中禽白血病病毒(ALV)污染情况,随机抽取安徽省鸡群使用的国内外公司(国内7家、国外1家)活毒疫苗(5个品种),先针对ALV各亚群保守的pol基因设计一对引物进行PCR检测,进一步针对env基因设计2对特异性引物,进行ALV J亚型和A^E亚型PCR检测。pol基因结果为鸡传染性囊病病毒(IBDV)B87株国内7个公司为阳性,鸡痘(AP)、鸡传染性喉气管炎(ILT)某国外公司为阳性,鸡新城疫(ND)LaSota株国内某公司为阳性。env基因结果为IBDV-B87株(国内某公司)和AP为J亚型阳性,ILT、ND-LaSota株和IBDV-B87株(国内另6个公司)为A^E亚型阳性。本研究共检测疫苗120份,ALV检出率达28.57%。因此,使用PCR法可作为活毒疫苗中ALV污染检测的有效方法,同时可以进一步鉴定污染的ALV是J亚型或A^E亚型。The research aimed to study on PCR Method for the detection of ALV pollution in live vaccines.Five kinds of live vac-cines that were produced by one foreign company and seven domestic companies were randomly sampled in Anhui Province.The prim-ers were designed according to pol gene which was conservative in every subgroup of ALV for PCR detection. Then two pair of prim-ers were designed according to env gene for ALV-J and ALV-A^E for further PCR detection.The positive results of pol gene wereIBD-B87（seven domestive companies）,AP and ILT（both produced by one foreign company）,ND（one domestive company）. The posi-tive results of ALV-J were IBD-B87（one domestive companies）and AP.The positive results of ALV-A^E were ILT、ND-LaSotaand IBD-B87（other six domestic companies）.One hundred and twenty samples were detected and the positive rate of ALV reached 28.57%. PCR method could be used as an effective method for detecting ALV pollution in live vaccine,which can further identifyALV-J and ALV-A^E.

关 键 词：活毒疫苗 禽白血病病毒 污染 检测 

分 类 号：S852.4[农业科学—基础兽医学]