中药复方应激康对热应激肉鸡HSP70m RNA表达影响的研究  

Effects of Compound traditional Chinese medicine(TCM)on Hsp70m RNA expression in heat stress chicken

作　　者：武果桃[1] 姜俊兵[2] 牛国庆 任杰[1] 孟冬霞[1] 赵娟[1] 牛琛[4] 

机构地区：[1]山西省农业科学院畜牧兽医研究所,山西太原030032 [2]山西农业大学动物科技学院,山西太谷030800 [3]太原市小店区畜禽繁育工作站,山西太原030032 [4]东北农业大学资源与环境学院,黑龙江哈尔滨150030

出　　处：《中国兽医杂志》2014年第9期50-53,共4页Chinese Journal of Veterinary Medicine

基　　金：山西省科技攻关项目(20120311023-3);山西省农业科学院科技攻关项目(2012YGG33)

摘　　要：为了研究复方中药对热应激肉鸡肝脏HSP70mRNA表达的调控效果;试验选取120只18日龄AA+肉仔鸡,随机分为4组,即复方中药组、维生素组、纯中药组和高温对照组进行试验,试验后每组迅速宰杀3只试验鸡,取肝脏组织进行HSP70mRNA表达的检测。结果表明:高温应激后,复方中药冲剂组和纯中药冲剂组鸡肝脏中HSP70 mRNA表达显著增加,分别比高温对照组、维生素组高2.98倍、3.28倍和4.66倍、5.13倍,说明本试验复方中药可通过上调HSP70 mRNA表达,提高机体热耐受水平,达到保护细胞作用。In order to study the effect of compound traditional Chinese medicine（TCM）on the thermal stress of Hsp70 mRNAexpression in chicken liver;120 AA ＋ broiler chickens at 18 days of age were selected and randomly divided into four groups,namelythe compound Chinese medicine group,vitamin group,the pure Chinese Medicine preparation group and the high temperature controlgroup. Three experimental chickens of each group were sacrificed quickly after the experiment,the liver tissue was taken for the detectionof HSP70 mRNA expression. The results show that under high temperature stress,HSP70 mRNA expression in experimental chickenliver of the compound Chinese medicine group and the pure Chinese Medicine preparation group of increased significantly,with 2.98 times and 3.28 times higher than that of the high temperature control group,4.66 times and 5.13 times higher than that of the vitamingroup,respectively. This compound Chinese medicine can increase the level of HSP70 mRNA,which in turn raise the body heat tolerance level and protect cell.

关 键 词：肉鸡 热应激 HSP70MRNA 表达 复方中药 

分 类 号：S858.31[农业科学—临床兽医学]