两株猪博卡病毒的检测及遗传演化分析  

Detection and genetic analysis of two strains of Porcine Bocavirus

作　　者：周宇[1] 唐连飞[2] 朱事康[1] 朱中武[2] 佟铁铸[1] 禹思宇[2] 刘星[1] 陈燕忠[1] 邹东辉[1] 罗卓军[1] 

机构地区：[1]惠州出入境检验检疫局技术中心,广东惠州516006 [2]湖南出入境检验检疫局,湖南长沙410004

出　　处：《中国兽医杂志》2014年第10期9-12,共4页Chinese Journal of Veterinary Medicine

基　　金：国家质检总局科技计划项目(2014IK243)

摘　　要：根据Gen Bank公布的猪博卡病毒序列,设计特异性引物,分别对猪博卡病毒（PBo V）的NS1基因、NP1基因、VP1/VP2全基因进行PCR方法测定,所得到的2个阳性样本分别命名为GD4和GD18。各基因遗传分析表明：GD4和GD18株各基因同源性较低,在34.9%~50.5%之间;猪博卡病毒主要分为3个大的分支,GD4和GD18分别处于不同的分支;分别与HQ223038和KF206167亲缘关系较近。According to the Gen Bank,the specific primers were designed to detect the PBo V NS1 gene,NP1 gene,and VP1/VP2 gene of PBo V.Two positive samples were named for GD4 and GD18 respectively.The genetic analysis indicated that the homologies of these two strains were 34.9%~50.5%,Pbo V includes three large branches,they were in different clusters.They indicate a close relationship with HQ223038 and KF206167 respectively.

关 键 词：猪博卡病毒 检测 遗传分析 

分 类 号：S852.651[农业科学—基础兽医学]