氟致大鼠肾脏组织形态结构损伤的观察  

The Observation of Structural Lesions of Rat Renal Tissue Induced by Fluoride

作　　者：袁建琴[1] 白峰[2] 张建海[2] 王俊东[2] 

机构地区：[1]山西农业大学生命科学学院,山西太谷030801 [2]山西农业大学动物科技学院,山西太谷030801

出　　处：《中国兽医杂志》2014年第10期25-26,29,I0004,共4页Chinese Journal of Veterinary Medicine

基　　金：国家自然科学基金资助项目(31172380);山西省高等学校教学改革项目(J2012026)

摘　　要：为了观察大鼠氟中毒肾脏病理变化特点,探讨过量氟对肾脏的毒性作用。选用60只1月龄的Wistar大鼠,随机分为4组:分别饲喂含0 mg/L、25 mg/L、50 mg/L和100 mg/L氟化钠的饮水5个月,并通过石蜡组织切片观察氟对肾脏的损伤作用。结果25 mg/L和50 mg/L氟中毒组大鼠肾脏肾小管排列结构无异常变化,肾小球细胞出现空泡样病变。100 mg/L氟中毒组大鼠肾脏肾小管排列结构没有明显的异常,肾小球内皮细胞和肾小管上皮细胞出现空泡样变,且近曲小管上皮细胞有脱落现象。25 mg/L、50 mg/L和100 mg/L氟中毒组肾小囊囊腔面积同对照组比较增大显著;肾小球球囊面积明显高于对照组,且随着染氟剂量的增加,肾小球球囊面积也相应的增加。表明,不同剂量的氟(25 mg/L、50 mg/L和100 mg/L)均可造成肾脏的损伤,且随着染氟剂量的增加,氟对肾脏的毒性作用也增加。本研究为日后建立合适剂量氟中毒大鼠动物模型和有效降低氟摄入量提供了参考依据。In order to analyze the toxic effect of fluoride on non-skeletal tissues,60 Wistar rats with age of a month old were selected,and randomly divided into 4 groups with 15 rats in each group.The animals were fed respectively with water containing 0 mg/L,25 mg/L,50 mg/L and 100 mg/L sodium fluoride.The lesions of renal tissue induced by fluoride were studied by paraffin-embedded tissue specimens.The results showed that the renal tubule had normal arrangement,but vacuolated changes were found within glomerular endothelium in 25 mg/L and 50 mg/L fluoride-treaded group;that the kidney tubule had normal situation as well as vacuolated lesions within glomerular endothelium and tubular epithelium,tubular epithelium were found in 100 mg/L fluoride-treaded group;that the kidney tubule had slight disarrangement,degeneration and necrosis of glomerular endothelium and tubular epithelium was observed.The size of bowman＇s capsule in 25 mg/L,50 mg/L and 100 mg/L fluoride was larger than that in treated group.In chronic fluorosis,the size of glomerular capsule,bowman＇s capsule and tubular cavity showed great damaging changes.The results suggested that different doses of fluoride（25 mg/L,50 mg/L and 100 mg/L）could cause kidney damage,and with the increase of fluoride doses,fluoride also increased toxic effects on the kidney.The study will provide references for establishing the appropriate dosages of fluorosis rat animal models and effectively reducing fluorine intake.

关 键 词：氟 大鼠 肾脏 损伤 观察 

分 类 号：S852.1[农业科学—基础兽医学]