基于动态光镊技术的卵巢癌SKOV3细胞凋亡检测  

Monitoring SKOV3 Cell Apoptosis Based on Dynamic Laser Tweezers

作　　者：张聿全 吴晓静[2] 王弋嘉[2] 闵长俊[3] 朱思伟[2] 袁小聪[3] 

机构地区：[1]南开大学现代光学研究所,天津300071 [2]天津市人民医院,天津300191 [3]深圳大学光电工程学院微纳光学研究所,广东深圳518060

出　　处：《中国激光》2014年第11期108-112,共5页Chinese Journal of Lasers

基　　金：国家自然科学基金(61138003;61377052;11204141);天津市自然科学基金(11JCZDJC15200);高等学校博士学科点专项科研基金(20120031120034)

摘　　要：化学疗法治疗癌症的主要机制是通过药物调控使无限增殖的癌细胞恢复到正常凋亡,因而实时检测用药过程中癌细胞的凋亡情况对于临床治疗具有重要意义。以卵巢癌SKOV3细胞作为研究对象,针对治疗药物(顺铂)不同浓度培养液的条件下,利用搭建的动态光镊系统在微流控芯片上通过测量捕获效率参数对四组细胞的凋亡情况分别进行检测。实验结果表明,该系统对不同药物浓度培养下SKOV3细胞凋亡情况的检测具有良好效果。与传统的检测手段相比,该系统细胞用量小、耗时短、无需标记、并可实现活体细胞检测,因此为细胞凋亡的临床检测提供了重要指导意义。Mechanism of chemotherapeutics for tumor therapy is to restore infinite proliferous cancer cells to normal apoptosis,therefore,apoptosis monitoring has important guidance for clinical treatment.A novel and robust method for apoptosis monitoring of tumor cells is engineered based on dynamic laser tweezers,using SKOV3 cell line as typical sample.SKOV3 cells are cultured under four gradient concentrations of cisplatin,and trapping efficiency of four groups are measured by proposed system,which presents great advantages of celerity,low consumption,and label-free explorations for living cells without perturbing experimental conditions in combination with classical probes.The proposed technique has great potential in improving cancer treatment by monitoring the objective efficacy of tumor cell killing.

关 键 词：生物光学 动态光镊 细胞凋亡检测 SKOV3 

分 类 号：O439[机械工程—光学工程]