化学比色法测定血清碳酸氢根的方法学研究  

Determination of Serum HCO_3^- Concentration by chemical Colorimetric Analysis

作　　者：刘瑞锁[1] 王克强[1] 付成燕[1] 张本文[2] 韩国新[1] 胡建功[1] 

机构地区：[1]泰山医学院附属医院检验科,山东泰安271000 [2]泰安市第一人民医院检验科,山东泰安271000

出　　处：《泰山医学院学报》2014年第10期1010-1012,共3页Journal of Taishan Medical College

摘　　要：目的建立一种新的血清碳酸氢根(HCO3-)测定方法。方法应用自配试剂建立两步终点测定法,可满足全自动和手工半自动测定,并对本方法进行方法学评价。结果本法比色液吸收峰556~558nm,线性范围1~50 mmol/L,批内CV 2.56%,批间CV 3.55%,回收率96~103%,与血气分析仪(X)相关良好,Y=0.48+0.989X,r=0.982(n=40),脂血、溶血、黄疸标本对结果无影响,健康人静脉HCO3-浓度x珋±s=25.76±2.26 mmol/L。结论本法操作简便,结果准确,试剂稳定,成本低廉,适合各级医院常规和急症使用。Objective：To establish a new method for the determination of serum HCO3 - concentration. Method Using self - made reagents to establish a test method of two - seep terminal point,which was methodotogically evaluated. Result The peak absorption was at 556 ~ 558nm,the linearity was from 1 ~ 50 mmol/ L,the intra - lot CV was 2. 56% ,the inter -lot CV was 3. 55% ,and the recovery rate was 96 ~ 103% . These resulte were in good correlation with those obtained by blood gasometry（x）：y = 0. 48 ＋ 0. 989x,r = 0. 982（n = 40）. Hyperlipoidemia,hemolysis and serions jaundice samples had no influence on the result,the density of HCO3 - in venous blood of heathy person x ＆#177; s = 25. 76 ＆#177; 2. 26 mmol/ L. Conclu-sions This method is a simple,accurate,and reliable,the reagents are stable and cheap. It is suitable for the routine and e-mergency determination of serum HCO3 - concentration for various hospitals.

关 键 词：化学比色法 碳酸氢根 二氧化碳分压 

分 类 号：R446.1[医药卫生—诊断学]