Effect of the chlortetracycline addition method on methane production from the anaerobic digestion of swine wastewater  

作　　者：Lu Huang Xin Wen Yan Wang Yongde Zou Baohua Ma Xindi Liao Juanboo Liang Yinbao Wu 

机构地区：[1]College of Animal Science, National Engineering Research Center for Breeding Swine Industry, South China Agricultural University [2]Ministry of Agriculture, Key Laboratory of Tropical Agricultural Environment, South China Agricultural University [3]Guangdong Enterprise Lab of Healthy Animal Husbandry and Environment Control [4]Nanhai Entry-Exit Inspection and Quarantine Bureau [5]Institute of Tropical Agriculture, Universiti Putra Malaysia,43400 Serdang, Malaysia

出　　处：《Journal of Environmental Sciences》2014年第10期2001-2006,共6页环境科学学报（英文版）

基　　金：supported by the National Natural Science Foundation of China (Nos. 30700586, 31072067, 31272481);the National Spark Program Project (No. 2012GA780001);the China Scholarship Council (NO. 201308440210)

摘　　要：Effects of antibiotic residues on methane production in anaerobic digestion are commonly studied using the following two antibiotic addition methods：（1） adding manure from animals that consume a diet containing antibiotics, and（2） adding antibiotic-free animal manure spiked with antibiotics. This study used chlortetracycline（CTC） as a model antibiotic to examine the effects of the antibiotic addition method on methane production in anaerobic digestion under two different swine wastewater concentrations（0.55 and 0.22 mg CTC/g dry manure）. The results showed that CTC degradation rate in which manure was directly added at 0.55 mg CTC/g（HSPIKE treatment） was lower than the control values and the rest of the treatment groups. Methane production from the HSPIKEtreatment was reduced（p 〈 0.05） by 12% during the whole experimental period and 15% during the first 7 days. The treatments had no significant effect on the pH and chemical oxygen demand value of the digesters, and the total nitrogen of the0.55 mg CTC/kg manure collected from mediated swine was significantly higher than the other values. Therefore, different methane production under different antibiotic addition methods might be explained by the microbial activity and the concentrations of antibiotic intermediate products and metabolites. Because the primary entry route of veterinary antibiotics into an anaerobic digester is by contaminated animal manure, the most appropriate method for studying antibiotic residue effects on methane production may be using manure from animals that are given a particular antibiotic, rather than adding the antibiotic directly to the anaerobic digester.Effects of antibiotic residues on methane production in anaerobic digestion are commonly studied using the following two antibiotic addition methods：（1） adding manure from animals that consume a diet containing antibiotics, and（2） adding antibiotic-free animal manure spiked with antibiotics. This study used chlortetracycline（CTC） as a model antibiotic to examine the effects of the antibiotic addition method on methane production in anaerobic digestion under two different swine wastewater concentrations（0.55 and 0.22 mg CTC/g dry manure）. The results showed that CTC degradation rate in which manure was directly added at 0.55 mg CTC/g（HSPIKE treatment） was lower than the control values and the rest of the treatment groups. Methane production from the HSPIKEtreatment was reduced（p 〈 0.05） by 12% during the whole experimental period and 15% during the first 7 days. The treatments had no significant effect on the pH and chemical oxygen demand value of the digesters, and the total nitrogen of the0.55 mg CTC/kg manure collected from mediated swine was significantly higher than the other values. Therefore, different methane production under different antibiotic addition methods might be explained by the microbial activity and the concentrations of antibiotic intermediate products and metabolites. Because the primary entry route of veterinary antibiotics into an anaerobic digester is by contaminated animal manure, the most appropriate method for studying antibiotic residue effects on methane production may be using manure from animals that are given a particular antibiotic, rather than adding the antibiotic directly to the anaerobic digester.

关 键 词：Chlortetracycline Methane production Psychrophilic anaerobic digestion Addition methods 

分 类 号：X713[环境科学与工程—环境工程]