呼吸道Hg、I、Mn和Sr染毒大鼠外周血中各元素含量的变化  

Changes of 4 trace elements in peripheral blood of rats by respiratory exposure

作　　者：孙璇[1] 张钰[1] 文华[1] 闫赖赖[1] 曾静[1] 解清[2] 王京宇[2] 

机构地区：[1]北京大学公共卫生学院,北京100191 [2]北京大学医药卫生分析中心

出　　处：《卫生研究》2014年第6期972-977,共6页Journal of Hygiene Research

摘　　要：目的探讨经呼吸道染毒后大鼠血液不同组分(全血、血浆、红细胞、外周血单核细胞)中Hg、I、Mn和Sr含量变化。方法将SD大鼠随机分成对照组和不同剂量受试组,采用4种元素(Hg、I、Mn、Sr)的混合溶液气管滴注染毒,连续染毒10天后股动脉取血。用Percoll液分离血液,分别收集血浆、红细胞、外周血单核细胞(PBMC),并用电感耦合等离子体质谱仪(ICP-MS)测定全血及血浆、红细胞、外周血单核细胞中4种染毒元素的含量。结果随着染毒剂量增加,全血与血浆中元素浓度均呈升高趋势;红细胞中Mn、Sr元素浓度各剂量组差异无统计学意义,Hg、I元素浓度均呈升高趋势;外周血单核细胞中Mn、I元素含量各剂量组差异无统计学意义,Hg元素含量呈升高趋势。结论全血和血浆中4种元素含量受到呼吸道染毒剂量的影响,而红细胞和PBMC中部分元素含量则较少受呼吸道染毒剂量影响。Objective To investigate the effects and changes of Hg, I, Mn and Sr in peripheral blood （whole blood, plasma, erythrocytes and peripheral blood mononuclear cells （PBMCs） ） of rats by inhalation exposure. Method 32 SD rats were divided randomly into 4 groups including one control group and three intervention groups. Each rat in three intervention groups was exposed respectively to three different doses of mixed elements （Hg, I, Mn, Sr） solution by intratraeheal instillation for 10 days. The whole blood of all rats were collected from arteria femoralis and separated into three parts by Percoll. The concentrations of 4 trace elements （Hg, I, Mn, Sr） in whole blood, plasma, erythrocytes and PBMCs were determined by inductively coupled plasma mass spectrometry （ICP-MS）. Result With the increasing of exposure doses, concentrations of 4 elements in whole blood and plasma tended to increase. No significant differences of Mn and Sr were found in erythrocytes （P 〉 O. 05 ）, while concentrations of I and Hg tended to increase with the increasing of exposure doses. No significant differences of Mn and I in PBMCs （P 〉 0.05） were found in the 4 groups, while concentration of Hg tended to increase with the increasing of exposure dose. Conclusion The elements concentrations in whole blood and plasma can reflect the influence of inhalation exposure, while the concentrations of some elements in erythrocytes and PBMCs are barely influenced by inhalation exposure doses.

关 键 词：微量元素 红细胞 外周血单核细胞 汞碘锰 

分 类 号：R994.6[医药卫生—毒理学] R446.11[医药卫生—药学]