糖尿病减弱心肌组织肌浆网钙转运三磷酸腺苷酶的小泛素样修饰  

Diabetes Reducing the Intensity of Sarcoplasmic Reticulum Ca^(2+)-ATPase-SUMOylation of Myocardium in Experimental Rats

作　　者：姚晶[1] 邵兴慧[1] 宋光远[1] 赵振燕[1] 高海洋[1] 武德崴 滕思勇[1] 吴永健[1] 

机构地区：[1]中国医学科学院北京协和医学院国家心血管病中心阜外心血管病医院心内科,北京市100037

出　　处：《中国循环杂志》2014年第11期932-936,共5页Chinese Circulation Journal

基　　金：教育部博士点基金(NO:20121106110010);协和医学院研究生创新基金(NO:2012-1002-30)资助

摘　　要：目的:探索糖尿病是否可以通过心肌组织肌浆网钙转运三磷酸腺苷(ATP)酶(SERCA2a)的小泛素样修饰进而影响SERCA2a的活性与表达。方法:采用小动物超声和左心室压力测定评价2型糖尿病大鼠(糖尿病组)和对照大鼠(对照组)心肌收缩和舒张功能;比较糖尿病组大鼠和对照组大鼠心肌组织内SERCA2a的表达情况,用免疫共沉淀方法以及小泛素样修饰试剂盒比较糖尿病组大鼠和对照组大鼠心肌组织内SERCA2a小泛素样修饰程度。结果:和对照组大鼠比较:糖尿病组大鼠心肌收缩、舒张功能均下降,尤以舒张功能下降更为明显;糖尿病组大鼠心肌组织内SERCA2a蛋白水平、基因表达和小泛素样修饰程度均下降;小泛素样修饰E2(Ubc9)的蛋白水平在糖尿病大鼠心肌中也下降,而小泛素样蛋白1(SUMO1)和小泛素样修饰E1(SAE1和SAE2组成的二聚体)蛋白水平无明显变化。结论:SERCA2a小泛素样修饰程度和Ubc9蛋白水平在糖尿病心肌组织中下降,提示SERCA2a小泛素样修饰和Ubc9与糖尿病的心肌损伤密切相关。Objective： To investigate the effect of diabetes on the intensity of sarcoplasmic reticulum Ca2＋-ATPase （SERCA2a）-SUMOylation and SERCA2a activity of myocardium in experimental rats. Methods： The 8 weeks old SD rats were divided into 2 groups, Diabetic group, with diet-induced type 2 diabetic rats and Control group, with normal rats. The systolic and diastolic cardiac functions were evaluated by echocardiography and left ventricular pressure measurement. The intensity of SERCA2a-SUMOylation was examined by co-immunoprecipitation and SUMOylation kit. Results： Compared with Control group, Diabetic group had decreased systolic and diastolic cardiac functions, especially for diastolic function;decreased SERCA2a protein expression and intensity of SUMOylation;decreased SUMOylation E2 （Ubc9 ） protein expression. The protein levels of SUMO1, SAE1 and SAE2 were similar between 2 groups. Conclusion： The intensity of SERCA2a-SUMOylation and Ubc9 decreased in diabetic myocardium which implies that SERCA2a-SUMOylation and Ubc9 were closely related to the damage of diabetic myocardium in experimental rats.

关 键 词：糖尿病 肌浆网钙转运ATP酶 小泛素样修饰 

分 类 号：R54[医药卫生—心血管疾病]