胃转流术对2型糖尿病大鼠血糖、胰岛素水平及胰岛数目的影响  

Effect of gastric bypass operation on blood glucose, insulin and islands of langerhans in rats with type 2 diabetes

作　　者：任泽强[1] 黄智龙 张蓬波[1] 张秀忠[1] 章红[3] 

机构地区：[1]徐州医学院附属医院普外科,江苏221002 [2]兰陵县人民医院普外科 [3]徐州医学院附属医院手术室,江苏221002

出　　处：《中华实验外科杂志》2014年第11期2437-2439,共3页Chinese Journal of Experimental Surgery

基　　金：江苏省徐州市科技计划资助项目（XM12B028）

摘　　要：目的 观察胃转流术（GBP）对2型糖尿病大鼠的降糖效果及对胰岛数目的影响.方法 健康雄性SD大鼠随机分为糖尿病手术组（DO组）、糖尿病假手术组（DS组）、糖尿病对照组（DC组）和正常手术组（NO组）,每组8只.术前及术后第1、2、4、8周分别测各组空腹血糖（FPG）、胰岛素（Fins）水平;术后2个月苏木素-伊红（HE）染色观察胰腺病理变化.结果 DO组术后第2周FPG由术前的（17.80±2.26） mmol/L降至（14.93 ±1.78） mmol/L（P＜ 0.05）,术后第8周降至（6.18 ±0.53） mmol/L（P ＜0.05）,DO组术后各时间点FPG明显低于DS、DC组相应时间点（P＜0.05）.DO组术后第8周Fins由术前的（11.89±0.87） mmoL/L升高到（14.70±1.04） mmol/L（P＜0.05）.术后病理可见平均胰岛数量,DO组比DC、DS组增加（P ＜0.05）;DO组胰岛较大,胰岛细胞密度增多.结论 GBP能明显降低2型糖尿病大鼠的血糖,提高胰岛素水平,与术后胰岛数目增多有关.Objective To observe the hypoglycemic effect and influence on islands of langerhans of gastric bypass operation in type 2 diabetes rats.Methods Healthy male SD rats were randomly divided into type 2 diabetes-operation group （DO group）,type 2 diabetes-sham operation group （DS group）,type 2 diabetes-control group （DC group） and normal-operation group （NO group） （n =8 each）.Plasma glucose concentration and insulin levels were measured respectively at fasting condition before,and 1 week,2 weeks,4 weeks and 8 weeks after operation.Pancreas pathological changes were observed two months after gastric bypass operation.Results The fasting blood glucose concentrations in DO group at the second week postoperation was decreased from （17.80 ± 2.26） to （14.93 ± 1.78） mmol/L （P 〈 0.05）,and those at 8th week postoperation decreased to （6.18 ±0.53） mmol/L （P 〈0.05）.The fasting blood glucose concentration in DO group was significantly lower than in DS and DC groups postoperation （P 〈 0.05）.The fasting plasma insulin levels in DO group were increased from preoperative （11.89 ± 0.87） to （14.70 ± 1.04） mmol/L （P 〈 0.05） at 8th week after gastric bypass operation.Postoperative pathologic examination revealed that average islands of langerhans in DO group were increased significantly as compared with DC and DS groups （P 〈 0.05）.In DO group,the islands of langerhans were obviously big,and the islet cells density increased.Conclusion Gastric bypass operation can markedly reduce plasma glucose concentrations in type 2 diabetes rats and increase fasting plasma insulin levels,which might be contributed to the increases in islands of langerhans after gastric bypass operation.

关 键 词：胃转流术 2型糖尿病 血糖 胰岛素 胰岛 

分 类 号：R587.1[医药卫生—内分泌]