不同贝母的指纹图谱鉴别研究  

The Identification of Different Fritillaria Species by Fingerprints

作　　者：闵会 吴健 胡江宁 叶剑锋 

机构地区：[1]浙江康恩贝药品研究开发有限公司,浙江杭州310052 [2]浙江康恩贝制药股份有限公司,浙江杭州310052

出　　处：《安徽农业科学》2014年第34期12051-12052,12070,共3页Journal of Anhui Agricultural Sciences

基　　金：国家科技支撑计划项目(2011BAI04B06);浙江省重点实验室和中试基地计划项目(2009E10009);浙江省企业研究院项目(2010E90011)

摘　　要：[目的]建立指纹图谱方法,区分与鉴别浙贝母、平贝母、川贝母药材。[方法]采用高效液相色谱法,蒸发光散射检测器。[结果]测定方法稳定可靠,3种贝母测定精密度、重复性RSD均小于5%,同时色谱峰均具有各自的特征性。[结论]该方法稳定可靠,可作为浙贝母、平贝母、川贝母区分与判定的有效途径。[Objective] To establish a fingerprint determination method to distinguish and identify Fritillariae thunbergii bulbus,Fritillariae cirrhosae bulbus,Fritillariae ussuriensis bulbus.[Method] HPLC and ELSD were adopted.[Result] The method is reliable,the reproducibility and precision of Fritillariae thunbergii bulbus,Fritillariae cirrhosae bulbus,Fritillariae ussuriensis bulbus are all less than 5％,and the chromatographic peaks are characterized.[Conclusion] This method is stable and reliable,it can be a good method to distinguish and identify Fritillariae thunbergii Bulbus,Fritillariae cirrhosae bulbus,Fritillariae ussuriensis bulbus.

关 键 词：浙贝母 平贝母 川贝母 HPLC-ELSD 指纹图谱 

分 类 号：S188[农业科学—农业基础科学]