芍药苷对乙醇诱导L02细胞的毒性和氧化损伤保护机制的影响  

Effect of paeoniflorin on toxicity and protection mechanism of oxidative injury of L02 cell induced by ethanol

作　　者：黄敏[1] 薄红兵[1] 王希[1] 王晓彬[2] 粟雪梅 向桢 甘学文[1] 石志艳[1] 

机构地区：[1]解放军第181医院整形外科,广西桂林541002 [2]南方医科大学附属桂林医院,广西桂林541002 [3]全军健康管理医学研究中心,广西桂林541002

出　　处：《中国临床药理学杂志》2014年第11期1030-1032,1038,共4页The Chinese Journal of Clinical Pharmacology

基　　金：广西自然科学基金资助项目(2011GXNSFA018309)

摘　　要：目的探讨芍药苷(PF)对乙醇诱导L02细胞细胞色素P450(CYP450)2E1表达及脂质过氧化的影响。方法用75 mmol·L-1乙醇体外诱导培养法建立L02细胞损伤模型,检测细胞培养液上清超氧化物歧化酶(SOD)和丙二醛(MDA)含量的变化,用定时定量-聚合酶链反应(RT-PCR)及细胞免疫化学技术观察芍药苷处理后的乙醇损伤L02细胞中CYP450 2E1的表达变化。结果与对照组比较,模型组血清SOD显著降低,MDA增高(P<0.05),CYP450 2E1mRNA及其蛋白表达明显增强(P<0.05);与模型组相比,芍药苷组SOD增高,MDA降低,CYP450 2E1 mRNA及其蛋白表达水平下降(P<0.05)。结论芍药苷对L02细胞乙醇损伤具有明显的改善作用,减轻脂质过氧化,其作用可能与调节CYP450 2E1的表达有关。Objective To investigate the effect of paeoniflorin （ PF） on cytochrome P450 2E1（ CYP450 2E1） and lipid peroxidation in L02 cell induced by ethanol.Methods L02 cell model was established by 75 mmol·L^-1 ethanol.After treated with paeoniflorin, superoxide dismutase （ SOD） and malondialdehyde （ MDA） levels in the cell culture supernatant were detected by biochemical analysis.The expressions of CYP450 2E1 in L02 cell were observed by RT-PCR and immunocyto-chemistry.Results Compared with the control group, serum SOD levels in model group were significantly decreased, while MDA levels were elevated （ P 〈0.05 ）.The levels of CYP450 2E1 mRNA and protein were significantly increased in model group （P〈0.05）.However, com-pared with the model group, SOD levels in the cell culture supernatant of PF group were much higher, while MDA levels were decreased, and the levels of CYP450 2E1 mRNA and protein were significantly decreased in PF group （ P〈0.05 ）.Conclusion The effect of PF on the improve-ment of L02 cell damaged by ethanol and reducing lipid peroxidation could be related with the regulation on the expression of CYP450 2 E1.

关 键 词：芍药苷 乙醇 L02细胞 细胞色素P450 2E1 脂质过氧化 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学] R971.1[医药卫生—基础医学]