RP-HPLC-PDA同时测定石见穿不同部位中5个三萜酸  

Determination of five triterpenic acids from Salvia chinensis of different parts by RP-HPLC-PDA

作　　者：姜琼 邹盛勤 

机构地区：[1]江西省天然药物活性成分研究重点实验室宜春学院化学与生物工程学院江西省高校应用化学与化学生物学重点实验室,江西宜春336000

出　　处：《中国中药杂志》2014年第22期4379-4382,共4页China Journal of Chinese Materia Medica

基　　金：国家高技术研究发展计划重点项目(2002AA2Z3217);江西省科技攻关计划项目(2007BG07200)

摘　　要：目的：建立RP-HPLC-PDA测定石见穿不同部位中5个三萜酸（坡模酸、山楂酸、科罗索酸、齐墩果酸和熊果酸）的定量方法。方法：采用Kromasil C18色谱柱（4.6 mm×250 mm,5μm）,流动相为乙腈-水（90∶10,体积比）,等度洗脱,检测波长205 nm,流速0.8 m L·min^-1,柱温28℃。结果：坡模酸、山楂酸、科罗索酸、齐墩果酸和熊果酸线性范围分别为0.096 00-2.400,0.123 0-3.075,0.242 0-6.050,0.283 0-7.075和0.273 0-6.825μg（r=0.999 8,0.999 7,0.999 9,0.999 5,0.999 9）,平均加样回收率（n=6）分别为98.43%,98.13%,100.6%,98.19%,99.15%（RSD 1.3%,0.67%,1.2%,0.87%,0.43%）。结论：该方法操作简便,重复性好,可用于石见穿中主要三萜酸的定量分析。Objective： To establish a RP-HPLC-PDA method for determination of five triterpenic acids（ pomolic acid,hawthorn acid,corosolic acid,oleanolic acid and ursolic acid） in different parts of Salvia chinensis. Method： The isocratic elution and separation was achieved on a Kromasil C18column（ 4. 6 mm × 250 mm,5 μm）,using acetonitrile-water（ 90∶ 10） as the mobile phase at a flow rate of 0. 8 m L·min^-1. The detection wavelength and column temperature were set at 205 nm and 28 ℃,respectively. Result： The calibration curves of pomolic acid,hawthorn acid,corosolic acid,oleanolic acid and ursolic acid were linear over the ranges of 0. 096 0-2. 400,0. 123 0-3. 075,0. 242 0-6. 050,0. 283 0-7. 075 and 0. 273 0-6. 825 μg（ r = 0. 999 8,0. 999 7,0. 999 9,0. 999 5,0. 999 9）,respectively. The average recoveries were 98. 43%,98. 13%,100. 6%,98. 19% and 99. 15% %,respectively,with RSD（ n = 6） being 1. 3%,0. 67%,1. 2%,0. 87% and 0. 43%. Conclusion： The proposed method is so simple and highly reproducible that it promises to be applicable for determination of major triterpenic acids in S. chinensis.

关 键 词：RP-HPLC 石见穿 三萜酸 坡模酸 山楂酸 科罗索酸 齐墩果酸 熊果酸 光电二极管阵列检测器 

分 类 号：R284.1[医药卫生—中药学]