Effects of Echinacea purpurea Polysaccharide on IEC-6 Cell Proliferation  

紫锥菊多糖对IEC-6细胞增殖的影响（英文）

作　　者：王晓珊[1] 史秋梅[1] 张艳英[1] 高桂生[1] 沈萍[1] 高光平[1] 梁敬玮 李艳云[1] 卢会朋 郭杨柳 吴楠[1] 

机构地区：[1]河北科技师范学院河北省预防兽医学重点实验室,河北昌黎066600

出　　处：《Agricultural Science & Technology》2014年第11期1876-1878,共3页农业科学与技术（英文版）

基　　金：Supported by National Natural Science Foundation of China(31472230);Natural Science Foundation of Hebei Province(C2014407068);Fund of Department of Science and Technology of Hebei Province(14966610D,12220408D);Fund from Hebei Provincial Department of Education for Hundreds of Outstanding Innovative Talents(II)(ZH2011244,Q2012037)~~

摘　　要：[Objective] This study was conducted to investigate the effects of Echi-nacea purpurea polysaccharides （EPS） on proliferation of rat intestinal epithelial cel IEC-6. [Method] The proliferation rate of IEC-6 cel s cultured in EPS at different concentrations and for different time was measured by MTT assay and analyzed by statistic methods. [Result] The proliferation rate of IEC-6 cel s cultured in EPS at al the concentrations and for different time was improved by different extents in com-parison with the control. In detail, 50 and 200 μg/ml EPS greatly improved the IEC-6 cel proliferation after 24 h of culture; then, the cel proliferation rate in the two treatments increased from 24 to 48 h, and declined from 48 to 72 h. The cel pro-liferation was also significantly improved by culturing in 100 μg/ml EPS for 72 h and in 500 μg/ml EPS for 48 h. After 48 h of culture, the proliferation rate of IEC-6 cel increased in a EPS dose-dependent manner. [Conclusion] EPS can promote IEC-6 cel proliferation, and thus improve the intestinal mucosal absorption and immune function of rat.[目的]研究紫锥菊多糖对大鼠小肠上皮细胞株IEC-6细胞增殖的影响。[方法]以IEC-6细胞为研究对象,采用MTT方法检测细胞增殖率,观察紫锥菊多糖对该细胞增殖的影响。[结果]紫锥菊不同剂量与不同的培养时间对IEC-6细胞的生长均有促进作用,但是随着处理时间的延长,不同浓度的促增殖效果也不尽相同,表现为在浓度为50、200μg/ml时增殖率随着处理时间的延长先增加后逐渐减弱,但在培养24 h后表现显著促增殖作用;浓度100μg/ml在72 h、浓度500μg/ml在48 h表现明显促增殖作用;经48 h处理的EPS其增殖率随浓度提高而增强。[结论]紫锥菊多糖具有促进IEC-6细胞增殖的作用,通过对小肠上皮细胞增殖的影响而发挥对肠道黏膜吸收和免疫功能的调整作用。

关 键 词：Echinacea purpurea  Polysaccharide  IEC-6 cel  Proliferation 

分 类 号：S852.4[农业科学—基础兽医学]