Identification and Biological Characteristics of Blank Spot Pathogen from Lilium lancifolium  

卷丹百合黑斑病病原鉴定及生物学特性研究（英文）

作　　者：吴金平[1] 丁自立[1] 刘晓艳[1] 郭凤领[1] 焦忠久[1] 邱正明[1] 

机构地区：[1]湖北省农业科学院,湖北武汉430064

出　　处：《Agricultural Science & Technology》2014年第11期1879-1882,共4页农业科学与技术（英文版）

基　　金：Supported by Modern Agricultural Industry Technology System Project of Ministry of Agriculture(nycytx-35-02-06);Key Program for Applied Basic Research of Agriculture of Hubei Provincial Science and Technology Plan Project(2012DBA07)~~

摘　　要：With the increasing planting area of Lilium lancifolium, the leaf fungal dis-ease of L. lancifolium is becoming more and more serious. In June and July of 2014, the excessive rainfal leads to the serious outbreak of leaf disease of L. lanci-folium. In mid-June, the wilting rate of L. lancifolium in seriously-infected field was even up to 50%-70%. In some fields, the shoots of L. lancifolium even al wilted. The pathogen was isolated from the infected leaves of L. lancifolium. Its pathogenici-ty, spore morphology, 18S rDNA sequence and biological characteristics were stud-ied. The results showed the isolated pathogen was Alternaria alternate. The lethal temperature of mycelial growth was 55 ℃. The optimum pH was 6-7. Among the tested carbon sources and nitrogen sources, the optimum carbon source was mal-tose, and the optimum nitrogen source was yeast extract.随着卷丹百合种植面积的扩大，卷丹百合叶部真菌性病害也越来越严重，尤其在2014年6~7月份，由于降水偏多，卷丹百合叶部病害大发生，6月中旬重病田卷丹百合枯死株率达50%~70%，部分田块卷丹百合地上部全部枯死。从卷丹百合发病叶片分离得到病原菌，进行致病性鉴定、孢子形态观察、18S rDNA序列分析，生物学特性研究，结果表明，该病原菌为半知菌链格孢（ Alternaria alternata）。该菌菌丝生长致死温度为55℃；适宜 pH为6~7；在供试的几种碳、氮源中，最适的碳源是麦芽糖，最适的氮源是酵母。

关 键 词：Lilium lancifolium  Black spot  Biological characteristic  Molecular identifi-cation 

分 类 号：S436.8[农业科学—农业昆虫与害虫防治]