Ambient Light Alters Gene Expression Pattern of Enzymes and Transcription Factors Involved in Phenylpropanoid Metabolic Pathway in Potato under Chilling Stress  

环境光照改变马铃薯苯丙烷代谢途径相关酶和转录因子基因对低温胁迫的应答模式（英文）

作　　者：秦玉芝[1] George Tai 谢开云[3,4] 何长征[1] 熊兴耀[1,3] 

机构地区：[1]湖南农业大学园艺园林学院,湖南长沙410128 [2]Potato Research Centre,Agriculture and Agri-Food Canada,Fredericton,New Brunswick E3B 4Z7,Canada [3]中国农业科学院蔬菜花卉研究所,北京100081 [4]国际马铃薯中心北京联络处,北京100081

出　　处：《Agricultural Science & Technology》2014年第11期1899-1904,共6页农业科学与技术（英文版）

基　　金：Supported by the Natural Science Foundation of China(31371683);the National Key Technology R&D Program of China(2012BAD02B05-8) during 12th Five-year Plan Period;Earmarked Fund for China Agriculture Research System(CARS-10-P19)~~

摘　　要：[Objective] Expressions of key enzymatic genes involved in phenyl-propanoid metabolic pathway in potato and StR2R3-MYB and StTGA transcripters were investigated in the present study. [Method] The primitive cultivar Yan was the materials for replicated trials and total RNA extracted from tissues of seedlings. Re-al-time florescent quantification PCR, multiple intervals of air temperature, light-il umi-nation and time-duration were factors of treatments in the experiment. Data on gene expressions were obtained and proceed to asses and compare effects based on statistical analysis. [Result] The results showed negative correlations between tem-perature degrees and expressions of StPAL, StDFR and StR2R3-MYB genes but not StTGA. Positive correlations, however, were derived between those of StCHS, StDFR and StR2R3-MYB and light-intensity. Significant interactive effects between expressions of StPAL and StDFR and treatments, light intensity and temperature degree, along the phenylpropanoid pathway were observed. Transcription regulator of StR2R3-MYB showed significant positive effect on the expression of StCHS of potato. StTGA transcription factor, on the other hand, gave significant negative ef-fects on the expression of StDFR. [Conclusion] Results from present study reveal the role of environmental factors and complicate interactions between such condi-tions as temperature-light il umination and mRNA function of target genes.[目的]通过实时荧光定量PCR分析不同环境温度和光照强度处理下,马铃薯苯丙烷类代谢途径关键酶基因,转录调节因子St R2R3-MYB和TGA基因表达情况。[方法]运用多因子及其互作逐步回归法建立光照强度、温度和处理时间影响基因表达的模式,逐步回归分析法研究St R2R3-MYB和TGA转录因子对苯丙烷类代谢途径关键酶基因的影响力。[结果 ]环境温度与St PAL、St DFR和St R2R3-MYB基因的表达量负相关,St TGA表达与温度正相关;环境光照强度与St CHS、St DFR和St R2R3-MYB表达正相关。环境光照强度和温度对St PAL和St DFR基因表达存在显著交互作用。[结论]环境光照强度影响马铃薯苯丙烷代谢途径关键酶St PAL和St DFR基因对温度的响应模式。马铃薯St R2R3-MYB转录调节因子对St CHS基因表达具有明显正向影响,而马铃薯St TGA转录调节因子对St DFR表达具有明显反向作用。

关 键 词：Potato  Light  Low temperature  Genes related to phenylpropanoid metabolic pathway  Regression analysis 

分 类 号：S532[农业科学—作物学]