CKLF1拮抗肽C19在小鼠特应性皮炎中的作用研究  

The study of chemokine-like factor 1( CKLF1 ) antagonism peptides C19 in mouse model with atopic dermatitis

作　　者：赵娟[1] 马晨丽 兰元翎 赵印梅[1] 殷兵[1] 贺钢枫[1] 杨高云[1] 

机构地区：[1]包头市中心医院皮肤科,内蒙古包头014000 [2]首都医科大学附属北京友谊医院皮肤科,北京100050 [3]大连医科大学基础免疫教研室,辽宁大连116000

出　　处：《临床和实验医学杂志》2014年第21期1748-1752,共5页Journal of Clinical and Experimental Medicine

基　　金：内蒙古自治区自然科学基金;项目编号:2011BS1108

摘　　要：目的探讨趋化素样因子1(CKLF1)拮抗肽C19局部皮下注射对特应性皮炎小鼠模型的作用。方法用卵清蛋白(OVA)经皮致敏BALB/c小鼠建立特应性皮炎模型。将60只BALB/c小鼠随机分成特应性皮炎组、对照组、预防组和治疗组,每组15只。观察小鼠致敏局部皮肤及组织病理改变。以ULSTM荧光标定技术检测各组总RNA中micro RNA的表现图谱。结果 1与对照组及预防组相比,皮炎组小鼠局部皮肤出现红斑、鳞屑等皮损,治疗组小鼠皮损经治疗后红斑、水肿减轻,鳞屑减少。小鼠局部皮损组织病理学显示,经CKLF1拮抗肽C19预防或治疗后,小鼠表皮增厚、细胞间水肿、真皮血管扩张充血及炎性细胞浸润均较轻。2以ULSTM荧光标定技术检测各组总RNA中micro RNA的表现图谱,通过鉴定各组间上调与下调的特定micro RNA基因,筛选调节炎症细胞因子特定靶基因表达的micro RNA,初步分析发现,经CKLF1拮抗肽C19局部外用治疗或预防后,小鼠皮损中部分调节炎症细胞因子特定基因表达的micro RNA上调。结论 CKLF1拮抗肽C19可能在预防或治疗特应性皮炎中具有重要作用,为了解及探索治疗特应性皮炎的多肽类药物提供了新途径。Objective To explore the function of chemokine- like factor 1（ CKLF1） antagonism peptides C19 in mouse model with atopic dermatitis. Methods The mouse model of atopic dermatitis were established by sensitization with ovalbumin（ OVA） through the skin. 60 BALB / c mice were randomly divided into atopic dermatitis（ AD） group,control group,prevention group,and treatment group,15 mice in each group. The gene expression profile of micro RNA in total RNA of every groups by ULSTMfluorescence detection calibration was detected. Results1 The AD group showed local inflammation in the skin compared with the control group and prevention group. Epidermal thickening and significant inflammatory cellular infiltration were observed and the skin lesion of treatment group mice improved after 2 times treatment with C19. 2 In order to detect the gene expression profile of micro RNA in total RNA of every groups by ULSTMfluorescence detection calibration,the data indicates that the expression level of micro RNA which regulate the specific gene of inflammatory cytokines in the lesions. The gene was up- regulated after treatment or prevention with C19. Conclusion Prevention or treatment with the CKLF1 antagonism peptides C19 may play an important therapeutic role in atopic dermatitis. Further study about the peptide may contribute to better understanding and exploring new anti- inflammatory medicine for the treatment of atopic dermatitis.

关 键 词：小鼠 特应性皮炎 CKLF1拮抗肽C19 MICRORNA 

分 类 号：R758.2[医药卫生—皮肤病学与性病学]