基于血红蛋白-石墨烯构建的过氧化氢传感器  

Construction of Hydrogen Peroxide Sensor Based on Hemoglobin-graphene

作　　者：翟牧牧 徐杰[2] 于伶俊 崔荣静[2] 谷宁[1] 

机构地区：[1]河北师范大学化学与材料科学学院,河北石家庄050024 [2]常熟理工学院化学与材料工程学院,江苏常熟215500

出　　处：《河北师范大学学报（自然科学版）》2014年第6期606-610,共5页Journal of Hebei Normal University：Natural Science

基　　金：国家自然科学基金(20905010);河北省自然科学基金(B2011205079);江苏省自然科学基金(BK20131191);苏州市科技发展计划(SYN201302)

摘　　要：用聚二甲基二烯丙基氯化铵（PDDA）将石墨烯（RGO）功能化,再与血红蛋白（Hb）超声混匀后滴涂到玻碳电极表面,用Nafion膜固定,得到Hb-PDDA-RGO/Nafion修饰的玻碳电极.研究了Hb的直接电化学,并构建了一种无媒介过氧化氢生物传感器.结果表明,该方法固定的Hb实现了直接电子传递,对过氧化氢的还原具有良好的电催化活性;构建的传感器对过氧化氢响应迅速,具有较宽的线性范围（0.1~350.2μmol/L）、较低的检出限（0.036 4μmol/L）、较高的灵敏度（30.61μA/（mmol·L-1·cm2））以及较低的米氏常数（18.1μmol/L）.Hemoglobin（Hb）was entrapped into graphene（RGO）that had been functioned with poly（diallyldimethylammonium chloride）（PDDA）under ultrasonic measurement.The direct electrochemistry of Hb was researched,and an unmediated biosensor for H2O2 reduction was developed by immobilizing Hb on aglassy carbon electrode modified with Hb-PDDA-RGO/Nafion.The results demonstrated that the immobilized Hb undergoes direct electron transfer and displays excellent bioelectrocatalytic activity for the reduction of H2O2.The sensor constructed by the Hb-PDDA-RGO/Nafion modified glassy carbon electrode displays an excellent response to the reduction of hydrogen peroxide with wider linear range from 0.1to350.2μmol/L,higher sensitivity with 30.61μA/（mmol·L-1·cm2）,lower detection limit with 0.036 4μmol/L and lower Michaelis constant with 18.1μmol/L.

关 键 词：石墨烯 血红蛋白 过氧化氢 生物传感器 直接电子传递 

分 类 号：O657.1[理学—分析化学]