24节气内乌鲁木齐10号泉水体细菌群落结构变化  

Structure changes of bacteria community in 24 solar terms at No.10 spring Urumqi

作　　者：薛娟[1,2] 张涛[1] 曾军[1] 李萍[1,3] 高小其[4] 娄恺[1] 

机构地区：[1]新疆农业科学院微生物应用研究所,乌鲁木齐830091 [2]石河子大学生命科学学院,石河子832000 [3]新疆大学生命科学与技术学院,乌鲁木齐830046 [4]新疆维吾尔自治区地震局,乌鲁木齐830011

出　　处：《生态学报》2014年第21期6214-6224,共11页Acta Ecologica Sinica

基　　金：新疆维吾尔自治区高技术研究发展项目(201216145)

摘　　要：为了解乌鲁木齐10号泉水体细菌群落结构及多样性在24节气内的变化,选取24节气当天采集泉水样品(计24份),利用末端限制性片段长度多态性(T-RFLP)技术对其进行群落结构及多样性分析,并结合该泉地球水文化学指标进行典型相关性分析。结果表明:泉水细菌群落的香农指数(H)变化幅度较大(1.088—3.003),春季(立春至谷雨)样品呈现出明显的波动趋势,并在惊蛰时达到最低值(1.088),夏季(立夏至大暑)和秋季(立秋至霜降)样品在节气内出现类似的递增趋势,冬季(立冬至大寒)样品则保持在较低水平(≈1.761);辛普森指数(D)和均匀度指数(E)在24节气内变化的差异不显著。24节气的泉水细菌群落组成主要有厚壁菌门、拟杆菌门、放线菌门、变形菌门及梭杆菌门,此外,还有少量隶属于蓝藻门、螺旋体门、异常球菌-栖热菌门、绿菌门、柔膜菌门和硝化螺菌门等的类群存在于某些特定的节气中;春夏季节革兰氏阴性菌为优势类群,秋冬季节,革兰氏阳性菌为优势类群;硫氧化菌类群(Acidithiobacillus、Thiothrix)和硫还原菌群落(Desulfobacter、Desulfoluna)的存在,与10号泉的含硫特性相关。通过对各项地球水文化学指标的分析显示,电导率、水汞、甲烷、硫化物在各节气的波动最为剧烈;乌鲁木齐10号泉水体细菌群落组成随24节气更替存在一定变化并与地球水文化学指标相关。Urumqi No.10 spring is located in the fracture zone of Willow ditch-Hongyanchi in southern Urumqi,and is one of the most important national key hydrological observation points.The spring water contains various geochemical elements,such as sulfide,methane,hydrogen,radon,sulfide,methane,carbon dioxide,helium,fluorine and mercury.Microbes living in the spring are intensely affected by hydro-geochemical changes caused by movements of the Earth＇s crust.The 24 solar terms,part of the Chinese traditional calendar,can accurately reflect changes in the Earth＇s climate,and the habitats of microorganisms also change with changes in the solar terms.In order to understand the changes in the structure of the bacterial community and diversity in Urumqi No.10 spring with the 24 solar terms,spring water samples collected during each period of the 24 solar terms were analyzed by T-RFLP,and CCA analysis combined with hydro-chemical analysis of indicators in the spring was conducted.Diversity analysis shows that the Shannon index（ H） of the spring bacterialcommunities has a large variation（ from 1.088 to 3.003）.Spring samples（ from ‘Beginning of Spring＇to ‘Grain Rain＇）show a significant fluctuating trend and reaches its lowest value in ‘Waking of Insects ＇（ 1.088）;in summer（ from‘Beginning of Summer＇to‘Greater Heat＇） and autumn（ from‘Beginning of Autumn＇to‘Frost＇s Descent＇）,the index shows similar increasing trends within the solar terms,while for winter（ from ‘Beginning of Winter＇to ‘Greater Cold＇）,the index remains at a low level（ approximately 1.761）.The changes in Simpson（ D） and evenness index（ E） were not significant throughout the 24 solar terms and the lowest value appeared in ‘Waking of Insects ＇.Hierarchical cluster analysis shows that the bacterial community patterns for the spring water appear to cluster with the solar terms,reflecting the similarity of the sample community composition between solar terms within the same season.Adjacent

关 键 词：24节气 细菌群落结构 多样性 T-RFLP 乌鲁木齐10号泉 

分 类 号：Q938.8[生物学—微生物学]