Cry1Ac和sck双价抗虫基因遗传转化甘蔗的研究  

Genetic Transformation of Cry1Ac and sck Gene into Sugarcane

作　　者：吴转娣[1] 吴才文[1] 曾千春[2] 苏火生[1] 刘新龙[1] 姚丽[1] 张敏[1] 刘家勇[1] 

机构地区：[1]云南省农业科学院甘蔗研究所云南省甘蔗遗传改良重点实验室,云南开远661699 [2]云南农业大学,云南昆明650201

出　　处：《热带作物学报》2014年第11期2236-2242,共7页Chinese Journal of Tropical Crops

基　　金：国家现代农业产业技术体系专项资金项目(No.CARS-20-1-1)

摘　　要：将双价抗虫基因,即修饰的苏云金芽孢杆菌毒蛋白基因(Cry1Ac)和修饰的豇豆胰蛋白酶抑制剂基因(sck)导入甘蔗优良品种ROC22,获得潮霉素抗性转化植株95株,35株能检测到外源抗虫基因,获得的13株RT-PCR双基因均呈阳性的甘蔗植株,斑点杂交检测有4株呈阳性。结果表明:外源抗虫基因已导入甘蔗基因组中。In the paper sugarcane, the cultivar ROC22 was genetically transformed with both CrylAc gene and sck gone, and selected with hygromycin B via agrobacterium-mediated transformation system. 95 transformed plantlets were obtained, and 35 of them showed positive by polymerase chain reaction. Furthermore, thirteen PCR-positive plantlets were detected by reverse transcription-polymerase chain reaction, all were positive. Thirteen of the RT- PCR-positive plants were taken for dot blot analysis, four of which were positive. The result showed that foreign genes were integrated into the sugarcane genome.

关 键 词：甘蔗 根癌农杆菌介导法 CRY1AC sck 遗传转化 

分 类 号：S688[农业科学—观赏园艺]