Pheomelanin Formation and Low Tyrosinase Activity in Fading Body Color Variant BdlR Strain Oryzias latipes  

作　　者：Nobuhiko Asada Aoi Kedamori Yumiko Kusano Tetsuro Takeuchi 

机构地区：[1]Department of Zoology, Faculty of Science, Okayama University of Science, Kita-ku, Okayama 700-0005, Japan [2]Faculty of Humanities, Shujitsu University, Higashi-ku, Okayama 703-8255, Japan

出　　处：《Journal of Life Sciences》2014年第6期517-521,共5页生命科学（英文版）

摘　　要：BdlR strain killifish Oryzias latipes, which can be found in natural populations in Kochi Prefecture, Japan, exhibit a fading body-color. The aim of this article is trace of body fade by enzymatic analysis especially blacking key enzyme tyrosinase. A single scale was collected from a single specimen of this strain at the dorsal axis, and the supernatant fi-action developed from this sample served as a tyrosinase extract. The extract fraction showed low tyrosinase activity when L-tyrosine, L-dopa and L-dopamine were used as enzyme substrates. After addition of L-cysteine to the sample scale, surface color gradually faded as pheomelanin formed. Tyrosinase was believed to have been in the folded phase and especially active arotmd the catalytic site and copper was detected in the scale. In hereafter, molecular analysis can attain above purpose.

关 键 词：Body-color PHEOMELANIN TYROSINASE BdlR Oryzias latipes. 

分 类 号：TQ658.2[化学工程—精细化工] TQ327.1