外周血特异性T淋巴细胞IFN-γ释放试验在辅助AIDS病人合并活动性TB感染诊断中的作用  

The diagnostic value of peripheral blood specific T-lymphocyte IFN-γrelease assay in active tuberculosis infection in AIDS patients

作　　者：刘丽改[1,2] 伦文辉[2] 赵红心[2] 肖江[2] 刘龙[2] 

机构地区：[1]北京市顺义区医院,北京101300 [2]首都医科大学附属北京地坛医院,北京100000

出　　处：《中国艾滋病性病》2014年第10期708-710,715,共4页Chinese Journal of Aids & STD

基　　金：十一五重大项目(2008ZX1005-003)~~

摘　　要：目的对怀疑合并结核(TB)的艾滋病(AIDS)病人的特异性外周血T淋巴细胞γ干扰素(IFN-γ)的检测结果,与临床及实验室诊断结果进行比较,探讨该方法在AIDS合并活动性TB中的辅助诊断价值。方法收集怀疑合并TB的AIDS病人的临床资料,对其TB患病情况进行综合分析。同时运用特异性外周血T淋巴细胞IFN-γ释放试验,对怀疑合并TB的AIDS病人进行检测。结果 22例AIDS病人中,确诊合并活动性TB的共9例(临床确诊TB 6例,实验室确诊TB 3例),酶联免疫斑点法(Elispot)阳性6例,其中临床确诊的活动性TB病人5例。暂未诊断活动性TB的11例病人中,7例Elispot阳性。结论特异性外周血T淋巴细胞IFN-γ释放试验,在AIDS合并活动性TB的诊断中具有重要价值。Objective To compare Elispot-IFN-γ results with clinical and laboratory diagnosis in suspected tu- berculosis （TB）, and to appraise the diagnostic value of Elispot-IFN-γ assay in active TB infection in AIDS patients. Methods Clinical data of AIDS patients suspected TB infection were collected and analyzed. Elispot-IFN-γ assay was performed. The assay results and clinical diagnosis were compared. Results Of 22 AIDS patients suspected TB infection, 9 were definitely diagnosed TB infection （six were clinically diagnosed and three were laboratorily diag- nosed）. Six subjects had positive Elispot results among which 5 were active. Of 11 inactive TB patients, 7 were Elispot positive. Conclusions It is clear that peripheral blood specific T lymphocyte IFN-γ release assay is valuable in diagnosis of active TB in AIDS patients.

关 键 词：特异性外周血T淋巴细胞IFN-γ释放试验 结核病 结核杆菌 艾滋病 

分 类 号：R512.91[医药卫生—内科学]