宁动颗粒对抽动秽语综合征模型大鼠纹状体5-羟色胺系统的影响  

Effect of “Ningdong Granules”on activity of 5-hydroxytryptamine system in rats with striatum of tourette syndrome

作　　者：侯广舜[1] 李安源[1] 赵林[1] 张峰[1] 王媛[1] 

机构地区：[1]山东大学附属省立医院中医科,山东济南250021

出　　处：《上海中医药杂志》2014年第11期74-77,共4页Shanghai Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(81273798);山东省自然科学基金资助项目(ZR2012HM030)

摘　　要：目的通过对抽动秽语综合征模型动物的行为学表现和纹状体5-羟色胺(5-HT)系统的检测,探讨宁动颗粒治疗抽动秽语综合征的作用机制。方法雄性Wistar大鼠50只,随机分为空白对照组(Control组)、模型组(IDPN组)、宁动颗粒低剂量组(NGL组)、宁动颗粒高剂量组(NGH组)和氟哌啶醇对照组(Hal组)。除空白对照组外,均采用亚氨基二丙腈(IDPN)腹腔注射制作抽动秽语综合征大鼠模型,观察动物行为学改变并评分。宁动颗粒组和氟哌啶醇对照组分别给予宁动颗粒和氟哌啶醇灌胃,空白对照组、模型组分别给予等量0.9%Na Cl溶液。干预8周后取脑纹状体,采用ABS-ELISA法测定各组5-HT及多巴胺(DA)含量,并用Real-time PCR技术测定5-羟色胺1A受体(5-HT1AR)、5-羟色胺转运体(SERT)mRNA的表达。结果宁动颗粒高、低剂量均可显著抑制模型大鼠的异常行为(P<0.01);与Control组相比,IDPN组大鼠纹状体内DA含量明显升高(P<0.01);各治疗组DA含量较IDPN组均明显下降(P<0.01)。与Control组相比,IDPN组大鼠纹状体内5-HT含量明显升高(P<0.01);NGH组和Hal组5-HT含量较IDPN组均明显升高(P<0.01)。IDPN组DA/5-HT比值较Control组明显升高(P<0.01);各治疗组DA/5-HT比值均明显低于IDPN组(P<0.01)。与IDPN组比较,NGL组和NGH组纹状体5-HT1AR和SERT mRNA的表达均明显降低(P<0.05,P<0.01)。结论 IDPN诱导的抽动秽语综合征模型大鼠存在纹状体5-HT系统功能反馈性上调,但其活性增强仍相对不足;宁动颗粒治疗抽动秽语综合征的作用机制与其促进纹状体内5-HT进一步合成、抑制其代谢及改善5-HT系统功能相对不足有关。Objective To clarify the mechanism of ＂Ningdong Granules＂ for Tourette syndrome （TS） by detecting the activity of 5- hydroxytryptamine system. Methods Fifty Wistar male rats were randomly divided into control group, model group （ IDPN group）, ＂Ningdong Granules＂ groups （ high, low dose） and haloperidol group. The TS model was established by 3,3＇ -iminodipropionitrile （IDPN） intraperitoneally. We observed the behavioral changes of the rats and given them scores. The ＂Ningdong Granules＂ groups and haloperidol group were treated by ＂Ningdong Granules＂ and haloperidol respectively. The blank control group and model group were treated by 0.9% NaC1. After 8 weeks intervention, we detected the contents of 5-hydroxytryptamine （5-HT） and DA by ABS-ELISA method, and we analyzed the 5-HT1A receptor （5-HT1AR） mRNA and serotonin transporter （SERT） mRNA expression by real-time PCR. Results Both the high and low dose of ＂Ningdong Granules＂ could obviously inhibit rat abnormal behavior （ P 〈 0.01 ）. Compared with the control group, the content of DA in the IDPN group was increased markedly （P 〈0.01 ） ; compared with the IDPN group, the DA contents in treatment groups were reduced markedly （P 〈0.01 ）. Compared with the control group, 5-HT level in the striatum of the IDPN group was increased markedly （P 〈0.01 ） ; compared with the IDPN group, 5-HT level in high dose of ＂Ningdong Granules＂ group and haloperidol group was increased markedly （P 〈0.01 ）. The ratio of DA/5-HT of the IDPN group was higher than that of the control group （P 〈 0.01 ）, and the ratio of DA/5-HT in treatment groups was lower than that of the IDPN group （P 〈 0.01 ）. Expression of 5-HTIA R and SERT mRNA in the striatum of the ＂Ningdong Granules＂ groups were reduced markedly （ P 〈 0.05, P 〈 0.01 ）. Conclusion The activity of 5- HT system wasfeedbackup-regulation in IDPN-induced TS rat models, but not enough. The mechanism of ＂ Ningdong Granules ＂ for

关 键 词：抽动秽语综合征 宁动颗粒 5-羟色胺系统 大鼠 

分 类 号：R285.5[医药卫生—中药学]