小鼠荧光层析成像系统及数据提取扩展方法  

Fluorescence Tomographical System and a Novel Data-ExtractionExtension-Method for Small Animal Imaging

作　　者：赵会娟[1,2] 王倩[1] 周晓青[1] 陈玮婷[1] 高峰[1,2] 

机构地区：[1]天津大学精密仪器与光电子工程学院,天津300072 [2]天津市生物医学检测技术与仪器重点实验室,天津300072

出　　处：《光子学报》2014年第10期6-12,共7页Acta Photonica Sinica

基　　金：国家自然科学基金(Nos.81271618;81101106;61108081);天津市自然科学基金重点项目(Nos.13JCZDJC28000;12JCQNJC09400)资助

摘　　要：基于电子倍增电荷耦合器件探测器搭建了面向小鼠活体非接触式测量的荧光扩散光层析成像系统,为了避免复杂的焦点矫正计算并降低逆问题的病态性,提出一种新的电荷耦合器件平板探测器信息提取及扩展方法,并根据信息提取方法发展了非接触式荧光扩散光层析成像图像重建算法.通过大量仿体荧光扩散光层析成像实验,证明当提取的信息对应于圆柱域投影的1/2、且将信息提取域分解为3个子域时重建可获得最好的效果.制备了皮下植入荧光目标体的活体小鼠进行荧光扩散光层析成像实验,与显微CT成像结果的对比表明:搭建的系统结合所发展的图像重建算法能够较好地重建出活体小鼠内荧光目标体的位置和浓度,有望应用到小鼠肿瘤模型的荧光层析成像中.A non-contact Fluorescence Diffuse Optical Tomography（FDOT）system based ElectronMultiplying CCD（EMCCD）was setup for in vivo imaging of mice.A novel Data-Extraction-ExtensionMethod（DEEM）was investigated to avoid the complex focus correction calculation for the optical lens and alleviate the ill-posedness of the inverse problem.Accordingly,a non-contact FDOT image reconstruction method based on the DEEM was developed.A lot of FDOT experiments were carried on the solid phantoms,which proved that the best reconstruction results can be achieved when the DataExtraction-area is half of the whole projection of the cylinder phantom on the CCD and is decomposed into three sub-areas.To further validate the method,the in vivo mouse with fluorescence target embedded was fabricated and the FDOT experiment was carried on.Reconstructed images from FDOT system and those from the Micro-CT demonstrate that the combination of the non-contact system and the proposed reconstruction method based on the DEEM can obtain the location and yield of the fluorescence target in the mouse correctly.The system can be further developed to detect the subcutaneous tumor of the small animals.

关 键 词：荧光层析成像 信息提取及扩展方法 小动物 皮下肿瘤成像 小动物成像荧光标记 

分 类 号：Q63[生物学—生物物理学]