绿咖啡豆中总绿原酸的纯化工艺研究  

Studies on the purification technology of total chlorogenic acid in green coffee beans

作　　者：王立志 胡晓梅 付其胜 田洪 

机构地区：[1]河北省天然色素工程技术研究中心,邯郸057250 [2]晨光生物科技集团股份有限公司,邯郸057250

出　　处：《中国食品添加剂》2014年第7期123-128,共6页China Food Additives

摘　　要：绿咖啡豆中的绿原酸共有九种同分异构体,所有同分异构体的总和称为总绿原酸,研究的主要目的是通过优化大孔吸附树脂纯化工艺将绿咖啡豆提取物中的总绿原酸含量由25%提高至70%以上,通过实验筛选合适的大孔吸附树脂,并优化树脂纯化的工艺参数如:上柱液浓度、上柱流速、解析溶剂度数、解析溶剂用量、解析流速等工艺指标,建立优化的纯化工艺路线。结果表明:上柱总绿原酸浓度为8mg/m L,上柱流速为1.0BV/h,解析用乙醇浓度为70%(v/v),乙醇用量为2BV,解析流速为1.5BV/h,经纯化后的总绿原酸经HPLC检测,总绿原酸含量可达71.23%,回收率可达87.46%。Chlorogenic acid in green coffee beans of has a total of nine isomers, all of the isomers together are called total chlorogenic acid. The main purpose of this study is through the optimization of purification process by macroporous adsorption resin, total chlorogenic acid content increased from 25% to 70% above. First, suitable macroporous adsorption resin was screened, then, the optimized purification process parameters such as concentration, flow velocity through the column, the column fluid solvent concentration and the amount were dertermined. Results showed that the total chlorogenic acid concentration on the column was 8 mg/mL, the velocity 1.0 BV/h, elude ethanol concentration 70% （v/v）, the amount 2 BV, ethanol analytic velocity of 1.5 BV/h. After purification, the total chlorogenic acid was detected by HPLC. The total chlorogenic acid content was up to 71.23% and the recovery rate was 87.46%.

关 键 词：绿咖啡豆 总绿原酸 大孔树脂 纯化 

分 类 号：R284.2[医药卫生—中药学]