酶联免疫吸附测定法与荧光定量 PCR 法在乙型肝炎病毒检测中的应用  

Enzyme-linked immunosorbent assay and fluorescence quantitative PCR method in the detection of hepatitis B virus

作　　者：刘辉 

机构地区：[1]湖北省京山县人民医院,湖北荆门431800

出　　处：《世界最新医学信息文摘》2014年第29期28-28,30,共2页World Latest Medicine Information Electronic Version

摘　　要：目的探讨ELISA法和QPCR法在乙型肝炎病毒检测中的效果。方法收集2012年7月至2013年1月在我院进行PCR技术HBV-DNA定量检测和ELISA法HBV-M检测的门诊及住院的乙型肝炎病毒患者或携带者309例，对比两种方法下乙型肝炎病毒的检测情况。结果ELISA法检测HBV-M的阳性率为99．0％，荧光定量PCR法检测HBV-DNA的阳性率为61．2％，差异有统计学意义（P〈0．05）。结论荧光定量PCR法对于乙肝病毒的测定较酶联免疫吸附测定法更为精确，灵敏度更高，对于指导临床、确定治疗方法更加有帮助，值得推广。Objective To investigate the effect of ELISA and QPCR method in the detection of hepatitis B virus. Methods From July 2012 to January 2013, 309 cases of outpatient and hospitalized patients with HBV or carriers of hepatitis B virus which were detected by quantitative HBV-DNA detection of PCR and HBV-M detection of ELISA were selected in our hospital. The results hepatitis B virus of detected by these two methods were compared.Results The positive rate of HBV-M detected by ELISA was 99.0% and positive rate of HBV-DNA detected by quantitative PCR was 61.2%. The difference was statistically significant （P 〈0.05）.Conclusion For detection of hepatitis B virus, quantitative fluorescence PCR method is more accurate than the enzyme-linked immunosorbent assay, with higher sensitivity. Therefore, it is more helpful to guide and determine clinical treatment, worthy of promotion.

关 键 词：酶联免疫吸附测定法 荧光定量PCR法 乙型肝炎病毒 

分 类 号：R446[医药卫生—诊断学]