圆锥铁线莲对慢性非细菌性前列腺炎的治疗作用及机制研究  

Protective Effects of Clematis terniflora DC. on Chronic Abacterial Prostatitis in Rats and Its Mechanism

作　　者：屠凌岚[1,2] 郑晓亮[1,2] 程丽艳[1] 颜冬梅[1,2] 郑高利[1] 

机构地区：[1]浙江省医学科学院药物研究所 [2]浙江省医学科学院分子医学中心,杭州310013

出　　处：《天然产物研究与开发》2014年第11期1761-1766,共6页Natural Product Research and Development

基　　金：浙江省医学重点学科群建设项目(XKQ-010-001);浙江省科技计划项目(2012F10005;2007R20009)

摘　　要：本文研究圆锥铁线莲水提取物对实验性慢性非细菌性前列腺炎的治疗作用,并对其抗炎的作用机制进行初步探讨。采用苯甲酸雌二醇致去势大鼠慢性非细菌性前列腺炎模型,研究圆锥铁线莲对慢性非细菌性前列腺炎的治疗作用。酶联免疫吸附测定法(ELISA)检测血清细胞因子白细胞介素1β(IL-1β)和肿瘤坏死因子α(TNF-α)的水平,Western Blot和免疫组化法检测前列腺组织环氧酶2(COX-2)和诱导型一氧化氮合酶(i NOS)的变化,探讨圆锥铁线莲发挥抗炎作用的可能机制。研究发现苯甲酸雌二醇致去势大鼠慢性非细菌性前列腺炎模型中,圆锥铁线莲2 g/kg组可见前列腺液中白细胞数明显降低,卵磷脂小体数量明显增加;病理切片可见炎症细胞浸润和成纤维细胞增生的情况有所改善。圆锥铁线莲能明显降低苯甲酸雌二醇致去势大鼠慢性非细菌性前列腺炎模型血清中IL-1β及TNF-α的含量,减少前列腺组织中COX-2和i NOS的表达。表明圆锥铁线莲水提取物对慢性非细菌性前列腺炎具有良好的抑制作用,其作用机制可能是通过调节炎症细胞因子水平、抑制COX-2以及i NOS的表达,从而避免局部产生过量的前列腺素(PG)和一氧化氮(NO),减轻局部炎性损伤实现的。To investigate the protective effects of Clematis terniflora DC. on chronic abactefial prostatitls （CAP） in rats and its possible mechanism. The CAP rat models were established by estrogen induction to observe the protective effects of Clematis tern/flora DC. The levels of IL-1/3 and TNF-ct in serum were evaluated by enzyme-linked immunosorbent as- say （ELISA） and the expression of iNOS and COX-2 were evaluated by Western Blotting and immunohistochemisty. The number of white blood ceils was obviously decreased, the density of lecithin corpuscle in prostatic secretion was increased and the edema,inflammatory i^ltration and proliferation of prostate were partly recovered by C. tern/flora in estradiol benzoate-induced chronic abaeterial prostatitis rats. C. terniflora can obviously reduce the level of IL-lfl and TNF-a in serum and the expression of iNOS and COX-2 in prostate tissue. These results demonstrated that C. terniflora had protec- tive effects on chronic abacterial prostatitis. Participation of immune regulation and inhibiting COX-2 and iNO$ expres- sion may be the possible mechanisms.

关 键 词：非细菌性前列腺炎 圆锥铁线莲 

分 类 号：R932[医药卫生—生药学]