大孔吸附树脂纯化无柄金丝桃茎部总黄酮工艺研究  

Optimization of Purification Conditions of Flavonoids from the Stem of Hypericum augustinii N. Robson by Macroporous Resin

作　　者：郭飞[1,2] 胡晓旭[1,3] 雷红菲 黄薇[1,2] 谭伟[1,2] 王红斌[1,2] 

机构地区：[1]云南民族大学民族药资源化学国家民委教育部重点实验室 [2]云南民族大学化学与生物技术学院,昆明650500 [3]大理市农产品质量安全检测站,大理671000

出　　处：《天然产物研究与开发》2014年第11期1853-1857,1863,共6页Natural Product Research and Development

基　　金：云南省大学生创新创业训练计划项目(201210691001);云南民族大学绿色化学与功能材料研究省创新团队项目(2011HC008);云南省高校绿色化学新能源与材料科技创新团队项目(2011UY09);大学生SRT创新性实验项目(2011HXZ01)

摘　　要：通过静态吸附筛选纯化无柄金丝桃茎部总黄酮的最佳树脂,并利用静态吸附解吸动力学确定纯化无柄金丝桃茎部总黄酮的工艺参数。实验结果显示AB8大孔吸附树脂为纯化总黄酮的最佳树脂。最佳工艺参数为:上样液浓度为1.30 mg/m L,体积为60 m L,p H=4.0,流速为1.00 m L/min,树脂柱径高比为1∶10,70%乙醇溶液(p H=7.0)为洗脱剂。经AB8树脂纯化,无柄金丝桃茎部总黄酮的纯度由30.26%提高到了55.70%,AB8大孔吸附树脂纯化无柄金丝桃茎部的总黄酮效果明显,其工艺参数简单可行。The optimal macroporous resin for the purification of flavonoids from the stem of Hypericum augustinii N. Rob- son was screened through static adsorption. The technological parameters of purification process were optimized using static adsorption and desorption kinetics. The results showed that AB8 was the best macroporous resin. The optimal tech- nological parameters were：the concentration of sample solution was 1.30 mg/mL,the sample volume was 60 mL,pH = 4.0 and the flow velocity was 1.00 mL/mln. The diameter height ratio of resin column was 1： 10,and the elution agents was 70% ethanol （ pH = 7.0）. The purity of flavonoids from the stem of H. augustinii increased from 30. 26% to 55.70% by AB8 macroperous resin. The developed purification process was simple and feasible.

关 键 词：无柄金丝桃茎 黄酮 大孔吸附树脂 

分 类 号：O629.9[理学—有机化学]