HPLC测定不同产地贝母中贝母辛的含量  

Determination of peimisine in Beimu from different growing areas by HPLC

作　　者：邹和平[1] 彭腾[2] 寇俊闯[2] 王小琴[2] 禹亚杰[2] 

机构地区：[1]太极集团有限公司,重庆401147 [2]成都中医药大学药学院教育部 中药材标准化重点实验室 中药资源系统研究与开发利用省部共建国家重点实验室培育基地,四川成都611137

出　　处：《中药与临床》2014年第3期4-5,16,共3页Pharmacy and Clinics of Chinese Materia Medica

摘　　要：目的:对8种不同产地贝母中贝母辛的含量进行测定。方法:采用Kromasil C18(4.6 mm×250mm,E76575)色谱柱,流动相为乙腈:0.2%二乙胺水溶液=40:60;流速:1.00mL/min;柱温:27℃;检测波长:205nm。测定了8种不同产地贝母中贝母辛的含量。结果:不同产地贝母中贝母辛含量有一定的差异,其中新疆贝母含量最高为0.02734%,伊犁贝母和康定贝母含量次之,其他5种贝母含量较少。结论:本方法灵敏度高,而且简便、准确、重现性好,可用于贝母的内在质量控制。Objective：To determine the content of peimisine in 8 kinds of Beimu from different growing areas.Method：The separation was performed on a Kromasil C18（4.6 mm×250mm） column,and the mobile phase was consisted of methanol and 0.2% diethylamine solution.The fl ow rate was 1.00 mL.min-1.Temperature was 27 ℃.The UV detection wavelength was 205 nm.Result：Peimisine contents were different in different Beimu.Beimu from Xinjiang had the highest content of peimisine（0.02734%）.The content of peimisine in Beimu from Yili and Kangding took the second place.The other 5 kinds of Beimu had less peimisine.Conclusion：The method is simple,accurate and can be used to control the internal quality of Beimu.

关 键 词：贝母 贝母辛 高效液相色谱法 

分 类 号：R284[医药卫生—中药学]