基于苯硼酸双羟基识别的竞争反应对黄芪甲苷的特异性检测研究  

Specific detection research of astragaloside Ⅳ based on competitive reaction of phenylboronic acid dihydroxy recognition

作　　者：李成林[1] 张琳[1] 洪金鑫 于妍妍[1] 

机构地区：[1]徐州医学院江苏省新药研究与临床药学重点实验室,徐州221004

出　　处：《中国当代医药》2014年第33期4-6,11,共4页China Modern Medicine

基　　金：国家自然科学基金资助项目(21205102)

摘　　要：目的创新性地提出一种可高灵敏、高选择性定量检测中药材中黄芪甲苷的新方法。方法将氨基苯硼酸共价吸附到多孔硅胶中,制备得到苯硼酸功能化的分子印迹材料。该印迹材料中的双羟基基团能与黄芪甲苷分子结构中的双羟基发生特异性识别。采用紫外分光光度法为检测手段,以茜素红（ARS）为光度指示剂,利用ARS与黄芪甲苷之间的双羟基竞争反应,观察加入不同浓度的黄芪甲苷后所引起可见光区吸光度的变化,达到高灵敏定量分析黄芪甲苷的目的。结果在510 nm处,溶液吸光度响应值的变化与黄芪甲苷的浓度在1×10^-6-6.4×10^-4mol/L内呈现良好的线性关系,线性回归系数为0.9929,检出限为0.3×10^-6 mol/L。结论本方法结果可信,重复性较好,可用于中药材中黄芪甲苷的定量分析。Objective To establish a new strategy for the sensitive and specific determination in Chinese medicinal herbs of astragaloside Ⅳ. Methods Molecular imprinting material phenylboronic acid functionalized was prepared by the adsorption of the amino phenylboronic acid covalently to porous silica gel.Specific recognition was produced between double hydroxyl groups in molecular imprinting material phenylboronic acid functionalized and two hydroxyl groups in the molecular structure of astragaloside Ⅳ.UV spectrophotometry was adopted as the mean of detection.ARS was adopted as an indicator for the photometric.The change of visible region added to different concentrations of astragaloside Ⅳ was observed to achieve objective of the high sensitive and quantitative analysis of astragaloside Ⅳ through the double hydroxyl competitive reaction between ARS and astragaloside Ⅳ. Results Under 510 nm,the absorbance displayed a linear relationship toward astragaloside Ⅳ in the concentration range from 1.0×10^-6 to 6.4×10^-4 mol/L with the linear regression coefficient of 0.9929 and the detection limit of 0.3×10^-6mol/L. Conclusion This method result is reliable.It has good reproducibility,can be used for quantitative analysis in Chinese medicinal herbs of astragaloside Ⅳ.

关 键 词：黄芪甲苷 茜素红 竞争反应 紫外分光光度法 

分 类 号：R286.0[医药卫生—中药学]