LPS刺激肥大细胞炎症因子分泌过程中miRNA的表达差异  

miRNA expression differences in inflammatory cytokines secretion in LPS-stimulated mast cells

作　　者：莫珍珍[1,2] 周瑶[1] 徐红[1] 刘峰[1] 赵德育[1] 

机构地区：[1]南京医科大学附属南京儿童医院呼吸科,江苏南京210008 [2]安徽马鞍山市十七冶医院儿科,安徽马鞍山243000

出　　处：《南京医科大学学报（自然科学版）》2014年第10期1317-1319,1341,共4页Journal of Nanjing Medical University(Natural Sciences)

基　　金：国家自然科学基金面上项目(81370132);南京市医学科技发展项目(201108012)

摘　　要：目的:研究在细菌脂多糖(lipopolysaccharide,LPS)刺激肥大细胞炎症因子分泌过程中miRNA的表达差异。方法:肥大细胞P815培养后,用LPS(1μg/ml)激发肥大细胞,16 h后终止反应,通过酶联免疫吸附实验(ELISA)检测上清中肿瘤坏死因子α(TNF-α)、白介素-6(IL-6)的水平。再收集细胞,通过实时荧光定量PCR法检测miRNA的改变。结果:在LPS刺激肥大细胞后,TNF-α、IL-6的分泌与基础分泌量相比明显升高,差异有统计学意义。同时在肥大细胞中干预组miRNA-126的表达是正常组的1.3倍(P<0.05),干预组miRNA-155的表达是正常组的1.2倍(P<0.05),干预组miRNA-223的表达是正常组的1.7倍(P<0.05),干预组miRNA-221的表达是正常组的1.2倍(P<0.05),干预组miRNA-192的表达是正常组的1.4倍(P<0.05)。结论:miRNA在炎症因子LPS刺激肥大细胞P815后存在差异性表达,而这些差异表达的miRNA可能与肥大细胞的功能调节存在相关性。Objective：To study the difference of expression of miRNAs of inflammatory cytokines in lipoplysaccharide（LPS）-stimulated mast cell secretion. Methods：P815 cells were stimulated by LPS（1 μg / ml） for 16 hours. The expression levels of TNF-αand IL-6 in supernatant were detected by ELISA. Real-time PCR was performed to detect the expression differcence of miRNAs after the cells were recollected. Results：In P815 after LPS stimulation,the secretions of TNF-α and IL-6 were largely up-regulated than those of control（P 〈 0.05）. Five miRNAs in the intervention group were found increasingly expressed compared with those in the control group,with miRNA-1261.3-fold,miRNA-155 1.2-fold,miRNA-223 1.7-fold,miRNA-221 1.2-fold,and miRNA-192 1.4-fold（P 〈 0.05）. Conclusion：miRNAs were expressed differentially in mast cells after LPS stimulation. The differcent expressions of miRNA may be invovled in mast cells function changes.

关 键 词：肥大细胞 MIRNA 炎症因子 

分 类 号：R392.1[医药卫生—免疫学]