维生素A对SD大鼠脑组织MC4R、POMC基因表达的影响  

Effect of vitamin A on appetite and gene expression of MC4R and POMC in rats

作　　者：李芹[1] 徐济达[2] 王嵘[3] 敖淑清[4] 张娟[4] 陆晓梅[4] 

机构地区：[1]南京医科大学附属南京妇幼保健院群体保健科,江苏南京210004 [2]南京医科大学康达学院,江苏南京210029 [3]南京医科大学基础医学院,江苏南京210029 [4]南京医科大学公共卫生学院,江苏南京210029

出　　处：《南京医科大学学报（自然科学版）》2014年第10期1342-1345,共4页Journal of Nanjing Medical University(Natural Sciences)

摘　　要：目的:探讨维生素A对SD大鼠食欲和黑皮素4受体(MC4R)、阿片黑素促皮质激素原(POMC)基因表达的影响,初步探讨维生素A影响大鼠食欲的机制。方法:将雄性SD大鼠随机分为A组(维生素A缺乏组)和B组(对照组)。喂养74 d,实验的第75天A组随机取出16只,分为A1组(维生素A缺乏组)和A2组(维生素A皮下补充组);B组随机取出8只,为B1组(对照组)。实验第79天,将这3组大鼠全部处死,取血和组织进行相关指标测定。A组剩余的动物按体重随机分为A3组(维生素A缺乏组)和A4组(维生素A食物补充组)。B组剩余的8只动物为B2组(对照组)。30 d后将其全部处死,取组织进行相关指标测定。结果:维生素A缺乏组进食量显著低于正常对照组,给予缺乏组大鼠维生素A皮下和食物补充后,大鼠进食量显著增多。维生素A缺乏组MC4R和POMC的mRNA表达升高,补充维生素A后大鼠POMC mRNA的表达水平明显降低。结论:维生素A影响大鼠食欲的可能机制是改变了下丘脑有关控制食欲的基因表达。Objective：To investigate the effects of vitamin A on appetite and the gene expression of MC4 R, POMC, and to explore the mechanism of vitamin A on rats’ appetite. Methods： Male Sprgue-Dawley（SD） rats were divided into two groups： group A（the vitamin A deficient group） and group B（the vitamin A normal control group）. Seventy-four days later, 16 rats were selected from group A and then divided into two groups： group A1（the vitamin A deficient group） and group A2（the vitamin A administration group）. Also 8 rats were selected from group B as group B1（the vitamin A normal control group）. At the 79 th day, all the rats in the three groups were sacrificed, and some relevant indices were measured. The remaining rats in group A were divided into two groups again： group A3（the vitamin A deficient group） and group A4（the vitamin A supplement group）. The remaining 8 rats in group B was group B2（the vitamin A normal control group）. All the rats were sacrificed after 30 days. Some relevant indices were also measured. Results： In the VAD group, the total food intake was significantly lower than that of the control group. After giving vitamin A supplement, the total food intake obviously increased. The expression of MC4 R and POMC in the VAD group was increased, while POMC was decreased obviously after vitamin A supplement. Conclusion： Vitamin A affects the expression of related appetite regulator, resulting in the change of food in take.

关 键 词：维生素A 大鼠 食欲 黑皮素4受体 阿片黑素促皮质激素原 基因表达 

分 类 号：R591.41[医药卫生—内科学]