普通野生稻miR160f的克隆和功能分析  

Clone and Function Analysis Mi R160f in Common Wild Rice(Oryza rufipogon Griff.)

作　　者：杨松楠[1] 王姣[2] 陈宗祥[2] 田新杰[1] 张静文[2] 龙艳[2] 裴新梧[2] 袁潜华[1] 

机构地区：[1]海南大学农学院,海口570228 [2]中国农业科学院生物技术研究所,北京100081

出　　处：《生物技术通报》2014年第11期114-118,共5页Biotechnology Bulletin

基　　金：国家转基因重大专项2014ZX08011-001;公益性行业(农业)科研专项经费项目(201403075);教育部热带作物新品种选育工程中心联合资助项目

摘　　要：MicroRNAs是一类在调节基因转录后表达中起重要作用的非编码RNA。miR160通过调节生长素响应因子(ARF)参与根细胞的分裂和分化,从而影响根的发育。克隆了普通野生稻mi R160f基因,并将其转入拟南芥中鉴定功能。结果表明,过表达mi R160f的拟南芥莲座叶的数量减少,抽薹时间缩短,开花的时间提前。qPCR检测显示miR160f的靶基因ARF10、ARF16及ARF17在过表达拟南芥植株中的表达下调,而ARF10和ARF16蛋白的缺失或减少会导致根冠细胞分化受阻、分裂失控,并导致根尖干细胞群的异位扩大,因此可以表明miR160不仅影响根的发育,还可能与水稻的开花时间相关。MicroRNAs are a class of non-coding RNAs involved in post- transcriptional control of gene expression. Former study on Arabidopsis thaliana reveals that miR160 involves in root cell division and differentiation by regulating auxin response factors（ARF）thus influence the root development. This study cloned miR160f gene from common wild rice and transferred intoArabidopsis thaliana to identify its function. The results showed that over-expression of miR160f decreased the number of rosette leaves, shortened bloting time, leading to early flowering. RT-PCR showed the expressions of gene ARF10,ARF16 and ARF17 are down-regulation caused by miR160f in transgenic Arabidopsis thaliana, while the deficiency of ARF10 and ARF16 protein can inhibit root cap cell differentiation, lose control of cell division and lead to ectopic expansion of apical stem cell populations Therefore, the result demonstrates that miR160 not only influence on root development, but also may influence flowering time of common wild rice.

关 键 词：花期 普通野生稻 拟南芥 表达载体 

分 类 号：S511.9[农业科学—作物学]