小豆锈病病原菌鉴定  

Identification of the pathogen causing rust on Adzuki bean

作　　者：支叶[1] 段灿星[1] 孙素丽[1] 王晓鸣[1] 朱振东[1] 

机构地区：[1]中国农业科学院作物科学研究所、农作物基因资源与基因改良国家重大科学工程,北京100081

出　　处：《植物病理学报》2014年第6期581-585,共5页Acta Phytopathologica Sinica

基　　金：国家现代农业产业技术体系建设专项(CARS-09)

摘　　要：小豆锈病是小豆的重要病害。然而，小豆锈病病原菌在我国还没有被鉴定。本文对从黑龙江省和内蒙古自治区采集的5个小豆锈病菌样品的分类地位进行了研究。形态学观察表明，小豆锈病菌夏孢子芽孔位于赤道线或赤道线上方，冬孢子平均壁厚为2．0μm。分子标记检测发现，小豆锈病菌不能被疣顶单胞锈菌特异性引物对UA—ITSF／UA—ITSR扩增，小豆锈病菌和豇豆锈病菌不能被豇豆单胞锈特异性引物对UV—ITSF／UV—ITSR区分。对豇豆单胞锈特异性引物对扩增的4个小豆锈病菌样品PCR产物进行测序，比对分析表明目标序列与小豆单胞锈ITS序列的同源性为99％～100％。基于夏孢子的芽孔位置、冬孢子壁的厚度、疣顶单胞锈菌及豇豆单胞锈特异性引物检测结果和ITS序列分析，小豆锈病菌被鉴定为小豆单胞锈。Rust is a severe disease on adzuki bean. However, the rust fungus has not been identified in China. Five rust isolates were collected from plants of adzuki bean in Heilongjiang Province and Inner Mongolia. The taxonomical status of these rust isolates was studied. Morphological examination revealed that position of urediniospore germ pores was equatorial or superequatorial, and the mean thickness of teliospore wall was 2.0 Ixm. Detection with specific molecular markers showed that a specific primer pair UA-1TSF/UA-1TSR for Uromyces apprendiculatus did not amplify any product in the five isolates, and the rust isolates from adzuki bean and cowpea could not be distinguished by U. vignae-specific primer pair UV-ITSF/UV-ITSR. The PCR products amplified by U. vignae-specific primer pair UV-ITSF/UV-ITSR from the four rust isolates were subjected to sequencing analysis. BLAST analysis of the resulting sequences showed that they were 99% to 100% identical to the ITS sequences of U. azukicola isolates. Based on the position of germ pores in urediniospores, the teliospore- wall thickness, amplification of U. appendiculatus- and U. vignae-specific markers, and ITS sequence analysis, the rust fungus on adzuki bean was identified as U. azukicola.

关 键 词：小豆 锈病 小豆单胞锈 

分 类 号：S435.21[农业科学—农业昆虫与害虫防治]