青霉菌灭活菌丝体诱导烟草BY-2悬浮细胞防卫反应的初步研究  

Primary researches on dry mycelium of Penicillium chrysogenum-induced defense responses of tobacco BY-2 cell suspensions

作　　者：钟宇[1,2] 陈壮壮[2] 谢虹[2] 燕飞[1] 时红敏 张鹏远[2] 王建光[2] 陈穗云[2] 

机构地区：[1]浙江省植物有害生物防控重点实验室--省部共建国家重点实验培育基地、浙江省农业科学院病毒学与生物技术研究所,杭州310021 [2]云南大学生命科学学院植物科学研究所,昆明650091

出　　处：《植物病理学报》2014年第6期679-686,共8页Acta Phytopathologica Sinica

基　　金：浙江省植物有害生物防控重点实验室开放基金(2010DS700124-KF1208);云南大学理(工)科校级基金(2010BY007);云南大学生命科学学院植物科学研究所青年教师基金(ZW201002);云南省烟草公司项目(2011YN60;2012YN10;2012YN36)

摘　　要：青霉菌灭活菌丝体(Dry Mycelium of Penicillium chrysogenum,DMP)是工业生产青霉素的残余副产物,研究发现DMP能够提高多种作物的抗病性。本文研究了DMP对烟草BY-2悬浮细胞防卫反应的诱导作用,并初步探索其诱导机制,结果表明:DMP处理烟草BY-2悬浮细胞后,产生了活性氧迸发,在处理后30 min达到峰值;DMP诱导烟草BY-2悬浮细胞胞外基质碱性化,该变化能被蛋白激酶抑制剂K252a部分抑制;苯丙氨酸解氨酶(PAL)和过氧化物酶(POD)的活性被诱导而明显升高,分别在处理后4 h和8 h达到峰值;DMP诱导了病程相关蛋白基因PR-1a、PR-1b以及抗病信号传导途径关键基因NPR1的表达。说明DM P能够诱导烟草BY-2悬浮细胞产生抗病防卫反应,其抗病信号可能是通过水杨酸信号途径进行传导的,蛋白质磷酸化参与了该抗病信号的传导过程。Dry mycelium of Penicillium chrysogenurn （DMP）, a waste product of the pharmaceutical industry, was shown to enhance pathogen-resistance in many crops. The effects of DMP on defense responses in tobacco BY-2 cell suspensions and the mechanism of DMP-induced resistance were studied. The defense responses elicited by DMP in tobacco BY-2 cells included an oxidative burst, extracellular alkalinisation, changes of phenylalanine ammonia lyase （PAL） and peroxidase （POD）activities, and expression of three defense-related genes PR-1a, PR-1b and NPR1. The amount of H2O2 reached the highest level at 30 min after DMP-treatment. Alkalinisa- tion response was induced by DMP in the cell suspensions, but reduced partially by protein kinase inhibitor K252a. The enzyme activities of PAL and POD increased quickly after DMP-treatment, and reached the highest level at 4 h and 8 h, respectively. Our data suggest that DMP could induce a series of defense responses in tobacco BY-2 cell suspensions, in which SA-dependent signal pathway and protein phosphorylation events may be involved.

关 键 词：烟草悬浮细胞 青霉菌灭活菌丝体 防卫反应 诱导抗性 信号传导途径 

分 类 号：S432.23[农业科学—植物病理学]