生物碱分子表面印迹聚合物材料的设计与制备及其分子识别特性  

Design and Preparation of Alkaloid Surface-Imprinted Material and Its Molecular Recognition Characteristics

作　　者：曹林交[1] 高保娇[1] 胡伟民[1] 

机构地区：[1]中北大学化学工程系,太原030051

出　　处：《应用化学》2014年第12期1390-1398,共9页Chinese Journal of Applied Chemistry

基　　金：山西省青年基金资助项目(2013021009-1)~~

摘　　要：采用巯基/偶氮二异丁腈(AIBN)表面引发体系,实现了甲基丙烯酸缩水甘油酯(GMA)在微米级硅胶微粒表面的引发接枝聚合,制得接枝微粒PGMA/SiO2;然后使接枝大分子PGMA的环氧基团与间二氨基苯磺酸钠(SAS)分子中的对位氨基发生开环反应,将苯磺酸钠基团键合在接枝大分子侧链,制得苯磺酸盐功能化的接枝微粒SAS-PGMA/SiO2。在对功能微粒SAS-PGMA/SiO2与苦参碱分子间的相互作用进行考察研究的基础上,以戊二醛为交联剂,实施了苦参碱分子的表面印迹,制备了苦参碱分子表面印迹材料MIP-SASP/SiO2。实验结果表明,在近中性溶液中,功能接枝微粒SAS-PGMA/SiO2与苦参碱分子间存在静电相互作用,以此为基础所设计与制备的表面印迹材料MIP-SASP/SiO2对苦参碱分子具有特异的识别选择性与优良的结合亲和性。相对于对照物金雀化碱而言,该印迹材料对苦参碱的识别选择性系数为10.7。The surface-initiated graft-polymerization of glycidyl methacrylate （GMA） on micron-sized silica gel particles was first conducted with a surface-initiating system of thiol group/azodiisobutyronitrile （ AIBN ） to obtain PGMA-grafted SiO2 particles. Sulfonate groups were bonded onto the side chains of PGMA by ring- opening reaction between the epoxy groups of PGMA and sodium 2,4-diaminobenzene sulfonate （ SAS ）, resulting in the formation of SAS-PGMA/SiO2 particles. The interaction between SAS-PGMA/SiO2 and matrine was examined. Surface-imprinting of the matrine molecule was performed with glutaraldehyde as the crosslinking agent to yield the matrine molecule surface-imprinted material MIP-SASP/SiO2. The experiment results show that in the nearly neutral solution, there is strong host-guest interaction between the functional grafted particles SAS-PGMA/Si02 and matrine. The surface-imprinted MIP-SASP/SiO2 particles, have specific recognition selectivity and excellent binding affinity for matrine. Relative to the cytosine control, the coefficient of recognition selectivity of the imprinted material MIP-SASP/SiO2 for matrine reaches 10. 7.

关 键 词：聚甲基丙烯酸缩水甘油酯 硅胶 分子表面印迹 苦参碱 分子识别 

分 类 号：O631[理学—高分子化学]